Browsing by Author "Kumari, H.M.P.S."

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    Determination of Effect of Pretreatment and Best Medium for Anther Culture for Selected Potato Varieties (Solanum tuberosum L.)
    (Uva Wellassa University of Sri Lanka, 2013) Chandrasoma, H.E.L.K.D.; Alwis, L.M.H.R.; Kumari, H.M.P.S.
    Potato (Solanum tuberosum L) is a starchy, tuberous crop and extensively cultivated in Nuwara Eliya and Badulla districts. Among the cultivated varieties in Sri Lanka, Granola was introduced variety and variety Golden Star was developed by Agriculture Research Station, Sitha Eliya. Vegetatively propagated potato using seed tubers are highly susceptible for diseases. Because anther culture can ensure that potato plants are disease-free, objectives were to determine the effective pretreatment, best medium and best performing variety for anther culture to develop a protocol for anther culture. Under glass house conditions during May to September, Golden Star and Granola are the only varieties produced flowers. Flower buds (4 to 6 mm long) of Golden Star and Granola were kept in refrigerator under 4 C for 24 hours and 6 C for 48 hours for pretreatment. Anthers were inoculated in six media in first stage. Number of contaminated petri dishes, number of days taken to anther enlargement, days taken to color change, difference of anther length, callus percentage and average diameter of calli were recorded after 8 weeks from explant establishment. In second stage after callus induction, developed calli will be transferred to a recommended regeneration media containing MS medium + 0.2mg L GA3 + 0.01mg L NAA. Number of days taken to calli regeneration, regenerated calli percentage and total number of shoots were recorded after 6 weeks from transferred. The lowest contamination percentage (16.6%) was observed on anthers pretreated at 4 C for 24 hours. Days taken to anther enlargement, days taken to color change and difference of anther length are not differed significantly.
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    Improvement of Micro-tuber Production Protocol for Potato Variety Granola To Increase Quality and Quantity of Micro-tubers
    (Uva Wellassa University of Sri Lanka, 2012) Gunasingha, K.R.S.S.; Alwis, L.M.H.R.; Kumari, H.M.P.S.; Samanthi, I.A.
    Potato (Solonuin luberosuin L.) is a starchy tuberous crop which belongs to the family solanaceae. Granola is a German potato variety with a high yielding and medium maturity and is recommended to grow in Sri Lanka by Department of Agriculture. In vitro induction of micro-tubers is a one type of micro propagation approach of potatoes. in vitro micro- tuberization of potato constitutes the transitory phase between in vitro multiplication end establishment of cultures in the field. They are considered as the miniature seed potatoes which represented an intermediary phase between "in vi/ro” plantlets and mini-tubers (Andreena & Champeanu, 2010). They are also called as the first generation of potsto seeds from tissue culture (Struik and Wiersena, 1999). /n vitro tuberization found to be controlled by a number of physical and chemical Factors including groMfi regulators, carbon source, photoperiod, culture conditions and genotype.(Hussey and Stacey, I984;Seabrook e/aI., 1993). Among those conditions, sucrose used as the carbohydrate and BAP (6- Benzylaminopurine) acts as the first generation synthetic cytokinins that elicit plant growth and development responses. Therefore, this study was carried out to improve the protocol for microtuber production to increase quality and quantity.