Determination of Effect of Pretreatment and Best Medium for Anther Culture for Selected Potato Varieties (Solanum tuberosum L.)
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Date
2013
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Publisher
Uva Wellassa University of Sri Lanka
Abstract
Potato (Solanum tuberosum L) is a starchy, tuberous crop and extensively cultivated in Nuwara
Eliya and Badulla districts. Among the cultivated varieties in Sri Lanka, Granola was introduced
variety and variety Golden Star was developed by Agriculture Research Station, Sitha Eliya.
Vegetatively propagated potato using seed tubers are highly susceptible for diseases. Because
anther culture can ensure that potato plants are disease-free, objectives were to determine the
effective pretreatment, best medium and best performing variety for anther culture to develop a
protocol for anther culture. Under glass house conditions during May to September, Golden Star
and Granola are the only varieties produced flowers. Flower buds (4 to 6 mm long) of Golden
Star and Granola were kept in refrigerator under 4 C for 24 hours and 6 C for 48 hours for
pretreatment. Anthers were inoculated in six media in first stage. Number of contaminated petri
dishes, number of days taken to anther enlargement, days taken to color change, difference of
anther length, callus percentage and average diameter of calli were recorded after 8 weeks from
explant establishment. In second stage after callus induction, developed calli will be transferred
to a recommended regeneration media containing MS medium + 0.2mg L
GA3 + 0.01mg L NAA. Number of days taken to calli regeneration, regenerated calli percentage
and total number of shoots were recorded after 6 weeks from transferred. The lowest
contamination percentage (16.6%) was observed on anthers pretreated at 4 C for 24 hours. Days
taken to anther enlargement, days taken to color change and difference of anther length are not
differed significantly.
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Keywords
Agriculture, Export Agriculture, Crop Production, Crop Production Technology, Vegetable Cultivation