Initiation of Callogenesis from Unfertilized Ovary Explants of Oil Palm (Elaeis guineensis Jacq.)

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dc.contributor.authorHerath, H.M.D.M.P.
dc.contributor.authorAlwis, L.M.H.R
dc.contributor.authorBandhupriya, H.D.D.
dc.date.accessioned2022-03-15T09:23:01Z
dc.date.available2022-03-15T09:23:01Z
dc.date.issued2013
dc.description.abstractOil palm is an efficient oil producing crop which has high economic value. Due to less capability of vegetative propagation, tissue culture is considered to be the only approach to produce clonal plantlets for oil palm. Several research based on somatic embryogenesis have been undertaken using various tissues (Euewens, 1976; Guedes et al., 2011) but no research work on oil palm ovary explants is reported. The objectives of this study were to find out relationship between maturity stages of inflorescence for callogenesis, to identify suitable in vitro culture media for callogenesis, and to determine the optimum 2, 4-D concentration for callus induction and multiplication in same callusing media. Methodology Unfertilized ovaries obtained from immature female inflorescences of oil palm (Elaeis guineensisJacq.) were tested as a source of explants for callogenesis. Inflorescence maturity stages of -2, -3 and -4 were cultured on Y3 (Eeuwens, 1976), CRI-72 (Karunarathne and Periypperuma, 1989) and OPC3 medium (Karun and Sajini, 1996) supplemented with 2,4-D levels of 160 µM, 180 µM and 200 µM. Collected female flowers were disinfected wit h 5% commercial bleach (chlorox) for 10 minutes followed by five rinses with sterile water. Ovaries were dissected out and cultured in vials containing 10 ml of callus induction medium under C in an incubation room until callus proliferation. Cultured ovaries were sub-cultured in to fresh medium (same callusing medium) and callus multiplication possibility was tested. Complete Randomized Design (CRD) with three factor factorial analysis was used as the experimental design.en_US
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/8514/07-PLT-Initiation%20of%20Callogenesis%20from%20Unfertilized%20Ovary%20Explants%20of%20Oil%20Palm%20%28Elaeis%20.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.subjectAgricultureen_US
dc.subjectExport Agricultureen_US
dc.subjectCrop Productionen_US
dc.subjectCrop Production Technologyen_US
dc.subjectPlantationsen_US
dc.subjectCoconuten_US
dc.subjectOil Palmen_US
dc.titleInitiation of Callogenesis from Unfertilized Ovary Explants of Oil Palm (Elaeis guineensis Jacq.)en_US
dc.title.alternativeResearch Symposium 2013en_US
dc.typeOtheren_US
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