Initiation of Callogenesis from Unfertilized Ovary Explants of Oil Palm (Elaeis guineensis Jacq.)
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Date
2013
Journal Title
Journal ISSN
Volume Title
Publisher
Uva Wellassa University of Sri Lanka
Abstract
Oil palm is an efficient oil producing crop which has high economic value. Due to less
capability of vegetative propagation, tissue culture is considered to be the only approach to
produce clonal plantlets for oil palm. Several research based on somatic embryogenesis have
been undertaken using various tissues (Euewens, 1976; Guedes et al., 2011) but no research
work on oil palm ovary explants is reported. The objectives of this study were to find out
relationship between maturity stages of inflorescence for callogenesis, to identify suitable in
vitro culture media for callogenesis, and to determine the optimum 2, 4-D concentration for
callus induction and multiplication in same callusing media.
Methodology
Unfertilized ovaries obtained from immature female inflorescences of oil palm (Elaeis
guineensisJacq.) were tested as a source of explants for callogenesis. Inflorescence maturity
stages of -2, -3 and -4 were cultured on Y3 (Eeuwens, 1976), CRI-72 (Karunarathne and
Periypperuma, 1989) and OPC3 medium (Karun and Sajini, 1996) supplemented with 2,4-D
levels of 160 µM, 180 µM and 200 µM. Collected female flowers were disinfected wit h 5%
commercial bleach (chlorox) for 10 minutes followed by five rinses with sterile water. Ovaries
were dissected out and cultured in vials containing 10 ml of callus induction medium under
C in an
incubation room until callus proliferation. Cultured ovaries were sub-cultured in to fresh
medium (same callusing medium) and callus multiplication possibility was tested. Complete
Randomized Design (CRD) with three factor factorial analysis was used as the experimental
design.
Description
Keywords
Agriculture, Export Agriculture, Crop Production, Crop Production Technology, Plantations, Coconut, Oil Palm