Functional Properties of Hydrolysates of Bioactive Peptides Extracted from Water Soluble Crude Protein from Hilsa kelee

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dc.contributor.authorMeepegamage, L.S.
dc.contributor.authorAbeyrathne, E.D.N.S.
dc.date.accessioned2022-09-22T06:47:09Z
dc.date.available2022-09-22T06:47:09Z
dc.date.issued2021
dc.description.abstractHilsa kelee also known as “Karattaya” is a marine pelagic, seasonal fish species which has a high catch during April to September. It is mainly used to produce fish meal and used as baits even though it has high nutrients. In addition, it has a low market value and a demand due to its bony structure leading to high wastage and production of fish protein hydrolysates (FPH) can be an alternative. 20 mg/ml concentrated water-soluble crude protein extractions were mixed with Protease from Bacillus licheniformis (60°C, pH 6.5-8.5), Papain from papaya latex (65°C, pH 5.8- 7.0), α-chymotrypsin from bovine pancreas (50°C, pH 7.8) and Elastase from porcine pancreas (37°C, pH 7.8-8.5) separately in 1:100 (enzyme: substrate) ratios. Each sample was incubated at different time intervals (0, 3, 6, 9, 12 and 24 hr) in predetermined optimum pH and temperatures. Then, 15% SDS-PAGE was used to determine the best hydrolysis condition. Bulk hydrolysates were prepared with Papain-6 h, Protease-3 h, α-chymotrypsin-3 h, Elastase-6 h followed with heat inactivation at 100°C for 15 miutes for further experiments. Then, TBARS assay, Ferrous chelation activity and antibacterial activities by agar well diffusion method was conducted. All treatments were triplicated (n=3). According to the results obtained from TBARS assay, FPHs from papain showed the highest antioxidant properties (5.84±0.05 MDA mg/L) compared to other treatments (p>0.05). Fe (II) chelation activity analysis revealed the FPHs produced from α-chymotrypsin showed the highest chelation (20.81±0.02%) (p<0.05). None of the hydrolysates showed any antimicrobial activities for tested microorganismas up to 20,000 ppm. Thus, FPHs produced using papain for 6 h followed with heat inactivation at 100°C for 15 miutes contain strong antioxidant activity Keywords: Hilsa kelee; Fish Protein Hydrolysates (FPH); Antioxidant; Metal Chelating; Antibacterial activityen_US
dc.identifier.isbn978-624-5856-04-6
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/9651/Page%20202%20-%20IRCUWU2021%20-%20204%20-%20L.S.%20Meepegamage%20-%20Functional%20Properties%20of%20Hydrolysates%20of%20Bioactive%20Peptides%20Extracted%20from%20Water%20Soluble%20Crude%20Protein.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.subjectFood Scienceen_US
dc.subjectFood Science and Technologyen_US
dc.subjectFood Technologyen_US
dc.subjectAnimal Sciencesen_US
dc.titleFunctional Properties of Hydrolysates of Bioactive Peptides Extracted from Water Soluble Crude Protein from Hilsa keleeen_US
dc.title.alternativeInternational Research Conference 2021en_US
dc.typeOtheren_US
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