Functional Properties of Hydrolysates of Bioactive Peptides Extracted from Water Soluble Crude Protein from Hilsa kelee
No Thumbnail Available
Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Uva Wellassa University of Sri Lanka
Abstract
Hilsa kelee also known as “Karattaya” is a marine pelagic, seasonal fish species which has a high
catch during April to September. It is mainly used to produce fish meal and used as baits even
though it has high nutrients. In addition, it has a low market value and a demand due to its bony
structure leading to high wastage and production of fish protein hydrolysates (FPH) can be an
alternative. 20 mg/ml concentrated water-soluble crude protein extractions were mixed with
Protease from Bacillus licheniformis (60°C, pH 6.5-8.5), Papain from papaya latex (65°C, pH 5.8-
7.0), α-chymotrypsin from bovine pancreas (50°C, pH 7.8) and Elastase from porcine pancreas
(37°C, pH 7.8-8.5) separately in 1:100 (enzyme: substrate) ratios. Each sample was incubated at
different time intervals (0, 3, 6, 9, 12 and 24 hr) in predetermined optimum pH and temperatures.
Then, 15% SDS-PAGE was used to determine the best hydrolysis condition. Bulk hydrolysates
were prepared with Papain-6 h, Protease-3 h, α-chymotrypsin-3 h, Elastase-6 h followed with heat
inactivation at 100°C for 15 miutes for further experiments. Then, TBARS assay, Ferrous chelation
activity and antibacterial activities by agar well diffusion method was conducted. All treatments
were triplicated (n=3). According to the results obtained from TBARS assay, FPHs from papain
showed the highest antioxidant properties (5.84±0.05 MDA mg/L) compared to other treatments
(p>0.05). Fe (II) chelation activity analysis revealed the FPHs produced from α-chymotrypsin
showed the highest chelation (20.81±0.02%) (p<0.05). None of the hydrolysates showed any
antimicrobial activities for tested microorganismas up to 20,000 ppm. Thus, FPHs produced using
papain for 6 h followed with heat inactivation at 100°C for 15 miutes contain strong antioxidant
activity
Keywords: Hilsa kelee; Fish Protein Hydrolysates (FPH); Antioxidant; Metal Chelating;
Antibacterial activity
Description
Keywords
Food Science, Food Science and Technology, Food Technology, Animal Sciences