Extraction of Crude Collagen from Yellowfin Tuna Skin (Thunnus Albacares) and Determination Functional Properties of its Hydrolysates

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dc.contributor.authorTHILANJA, G.P.D. D.S.
dc.date.accessioned2021-10-12T08:35:07Z
dc.date.available2021-10-12T08:35:07Z
dc.date.issued2014
dc.description.abstractCollagen is dominant protein in connective tissues and highly valuable in food industry. Fish processing by products are good alternative source for collagen. The objective of this study was to develop a simple non-toxic method to extract collagen from Yellowfin tuna skin and to check functional properties of its hydrolysats. Extraction procedure were conducted using acetic acid and citric acid with 0.5M concentrations. Based on 8% SDS- PAGE gel, type I collagen were identified. Enzymatic hydrolysis were done with protease, trypsin, pepsin enzymes with different time combinations (Oh, 3h, 6h, 9h, 12h, 24h) at 37°C after adjusting to its optimum pH level. Best hydrolysate were selected and subjected to antioxidant activity by using Diphenyl-1-picryhydrazyl (DPPH) radical scavenging activity and thiobarbituric acid reactive substances (TBARS) assay. Metal (Fe') chelating activity were evaluated by using frrrozine method and Antimicrobial activity were evaluated by using agar well diffusion method. Proximate analysis were conducted for raw skin to determine moisture, ash, protein, fat content and 59.4410.013%, 1.9110.37%, 28.5511.19%, 6.8310.30% values were obtained respectively. Hydrolysates produced after incubating for 0 h at 37°C followed with heat inactivation was selected as the best. Hydrolyzed produced using citric acid showed lower scavenging activity (63.62%) compared to acetic acid (85.07%) (p<0.05),In TBARS assay citric acid shows high antioxidant activity than acetic acid (p<0.05). In both acetic arid citric extractions Fe" chelating activity did not showed significant difference among the treatments (p>0.05),In agar well diffusion method it shows good antimicrobial activity with acetic acid than citric acid (p<0.05). According to the collagen hydrolysates incubated at 0 h at 37°C showed good antioxidant activity with acetic acid extraction with pepsin enzyme. This conclude that collagen hydrolysates produced using acetic acid and pepsin showed good antioxidant activity comparing with the ascorbic acid as positive control and it could be deserved to use as good alternative source as natural anti-oxidant in food industry. Key words: Fish collagen, Yellow fin tuna, Antioxidant activity, Hydrolysatesen_US
dc.identifier.otherUWU/ANS/14/0039
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/7252/ANS%2014%20039-17022021134128.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.relation.ispartofseries;UWU/ANS/14/0039
dc.subjectAnimal Science Degree Programme (ANS)en_US
dc.titleExtraction of Crude Collagen from Yellowfin Tuna Skin (Thunnus Albacares) and Determination Functional Properties of its Hydrolysatesen_US
dc.title.alternativeResearch Article – ANS 2014en_US
dc.typeThesisen_US
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