Extraction of Crude Protein from Hilsa kelee and Determination of its functional properties
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Date
2020
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Publisher
Uva Wellassa University of Sri Lanka
Abstract
Karattaya (Hilsa kelee) is a marine pelagic species. H. kelee is a seasonal fish species and
has high catch from April to September. However, it does not have a high market demand
due to its bony structure and sharp tough bones which make it hard to eat. Due to that, it
generates a large quantity of waste and currently used only in the production of fish meal.
Therefore, the objective of this study was to develop a simple nontoxic method for
extracting crude protein from H. kelee and to determine the functional properties of the
extract. Collected fish samples were blended and treated with 04 different ratios as a
sample: distilled water 1:1, 1:2, 1:3 and 1:4 to separate sarcoplasmic proteins followed
with salt extraction with 5%, 10%, 15% and 20% (w/v) concentrated NaCl at 1:10 ratio to
separate myofibrillar proteins separately. Crude extraction was lyophilized and selected
the best ratio and concentrate by using the yield and SDS-PAGE analysis. Antimicrobial
properties, antioxidant properties, and metal chelating properties of selected extracted
crude protein were determined. All treatments were replicated (n=3). H. kelee contained
moisture, ash, crude protein 67.39 ± 2.23%, 5.07. ± 0.92% and 20.38 ± 2.38%,
respectively. In yield analysis of water extraction 1:4 ratio showed the highest yield
compared to other treatments (p<0.05). In salt extraction, there was no significant
difference between yields (p>0.05), therefore 5% (w/v) was selected as the best sample
for further experiment. Antioxidant activities of extracted Fish Protein Concentrates
(FPC) were higher in the salt extracted sample than the water extracted sample (p<0.05).
FPC had a negative response to the Fe2+ chelating activity. No inhibition was observed
against E. coli and Total Plate Count (TPC) up to 20,000 ppm concentration in all FPC. It
concludes that extracted FPC contains strong antioxidant properties. However, they do
not exhibit Fe2+ chelating properties and antimicrobial properties.
Keywords: Hilsa kelee, Fish Protein Concentrate, Extraction, Antioxidant property
Description
Keywords
Agriculture, Animal Sciences, Fisheries