Extraction of Crude Protein from Hilsa kelee and Determination of its functional properties

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dc.contributor.authorJayathissa, R.C.M.
dc.contributor.authorRupasinghe, R.A.
dc.contributor.authorAbeyrathne, E.D.N.S.
dc.date.accessioned2021-01-08T04:22:38Z
dc.date.available2021-01-08T04:22:38Z
dc.date.issued2020
dc.description.abstractKarattaya (Hilsa kelee) is a marine pelagic species. H. kelee is a seasonal fish species and has high catch from April to September. However, it does not have a high market demand due to its bony structure and sharp tough bones which make it hard to eat. Due to that, it generates a large quantity of waste and currently used only in the production of fish meal. Therefore, the objective of this study was to develop a simple nontoxic method for extracting crude protein from H. kelee and to determine the functional properties of the extract. Collected fish samples were blended and treated with 04 different ratios as a sample: distilled water 1:1, 1:2, 1:3 and 1:4 to separate sarcoplasmic proteins followed with salt extraction with 5%, 10%, 15% and 20% (w/v) concentrated NaCl at 1:10 ratio to separate myofibrillar proteins separately. Crude extraction was lyophilized and selected the best ratio and concentrate by using the yield and SDS-PAGE analysis. Antimicrobial properties, antioxidant properties, and metal chelating properties of selected extracted crude protein were determined. All treatments were replicated (n=3). H. kelee contained moisture, ash, crude protein 67.39 ± 2.23%, 5.07. ± 0.92% and 20.38 ± 2.38%, respectively. In yield analysis of water extraction 1:4 ratio showed the highest yield compared to other treatments (p<0.05). In salt extraction, there was no significant difference between yields (p>0.05), therefore 5% (w/v) was selected as the best sample for further experiment. Antioxidant activities of extracted Fish Protein Concentrates (FPC) were higher in the salt extracted sample than the water extracted sample (p<0.05). FPC had a negative response to the Fe2+ chelating activity. No inhibition was observed against E. coli and Total Plate Count (TPC) up to 20,000 ppm concentration in all FPC. It concludes that extracted FPC contains strong antioxidant properties. However, they do not exhibit Fe2+ chelating properties and antimicrobial properties. Keywords: Hilsa kelee, Fish Protein Concentrate, Extraction, Antioxidant propertyen_US
dc.identifier.isbn9789550481293
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/5568/proceeding_oct_08-33.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.relation.ispartofseries;International Research Conference
dc.subjectAgricultureen_US
dc.subjectAnimal Sciencesen_US
dc.subjectFisheriesen_US
dc.titleExtraction of Crude Protein from Hilsa kelee and Determination of its functional propertiesen_US
dc.title.alternativeInternational Research Conference 2020en_US
dc.typeOtheren_US
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