INDUCTION OF EMBRYOGENIC CALLUS IN GRAPES (Vitis vinifera)

Simple item page
dc.contributor.authorGUNASINGHE, U.L.D.M.S.
dc.date.accessioned2021-04-21T09:06:27Z
dc.date.available2021-04-21T09:06:27Z
dc.date.issued2015
dc.description.abstractIn vitro propagation of Grapes (Vitis vinifera) extends opportunities for increasing plant material for cultivation. This experiment was conduct to develop an efficient protocol for surface sterilization and callus inducton from different explants of grapes. Grape peduncle segments and fruit skins were used as explants. Different Clorox concentrations and different soaking times were used to select the best surface sterilization method. The sterilized explants were cultured on MS medium supplemented with different hormone concentrations and combinations of NAA (Naphthalene Acetic Acid)-BAP (6- Benzyl Amino Purine), NAA- KIN (Kinetin), 2, 4—D (Dicholoro Phenoxy Acetic Acid) — BAP and 2, 4-D — KIN to investigate the effect on callus induction. Cultures were maintained at 25±2 °C temperature under dark conditions in a culture room. The best surface sterilization of peduncle was achieved with 25% Clorox for 20 minutes and best surface sterilization of fruit skins was achieved with 2.5% Clorox for 1 second. Peduncles are best explant for high frequency callus induction than the fruit skins. Among the NAA and 2, 4 —D hormones, NAA is better for callus induction from the peduncles and 2, 4 — D. is better for callus induction from the fruit skins. 10mgL-1 NAA + 0.5mgL-1 BAP are best hormone combination for earlier callus formation and to obtain higher volume callus from grape peduncles. Callus induction of grapes using peduncles and fruit *skins can be accomplished successfully using above methods of surface sterilization and culture establishment can be used successfully. Key words: Callus induction, Peduncles, Fruit Skins, NAA, BAP, KIN, 2, 4-Den_US
dc.identifier.otherUWU/EAG/11/0007
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/6533/UWULD%20EAG%2011%200007-14052019091430.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.relation.ispartofseries;UWU/EAG/11/0007
dc.subjectExport Agriculture Degree Programme (EAG)en_US
dc.titleINDUCTION OF EMBRYOGENIC CALLUS IN GRAPES (Vitis vinifera)en_US
dc.title.alternativeResearch Article – EAG 2015en_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
UWULD EAG 11 0007-14052019091430.pdf
Size:
2.76 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Export Agriculture Degree Programme (EAG)