INDUCTION OF EMBRYOGENIC CALLUS IN GRAPES (Vitis vinifera)
No Thumbnail Available
Date
2015
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Uva Wellassa University of Sri Lanka
Abstract
In vitro propagation of Grapes (Vitis vinifera) extends opportunities for increasing plant material for cultivation. This experiment was conduct to develop an efficient protocol for surface sterilization and callus inducton from different explants of grapes. Grape peduncle segments and fruit skins were used as explants. Different Clorox concentrations and different soaking times were used to select the best surface sterilization method. The sterilized explants were cultured on MS medium supplemented with different hormone concentrations and combinations of NAA (Naphthalene Acetic Acid)-BAP (6- Benzyl Amino Purine), NAA- KIN (Kinetin), 2, 4—D (Dicholoro Phenoxy Acetic Acid) — BAP and 2, 4-D — KIN to investigate the effect on callus induction. Cultures were maintained at 25±2 °C temperature under dark conditions in a culture room.
The best surface sterilization of peduncle was achieved with 25% Clorox for 20 minutes and best surface sterilization of fruit skins was achieved with 2.5% Clorox for 1 second. Peduncles are best explant for high frequency callus induction than the fruit skins. Among the NAA and 2, 4 —D hormones, NAA is better for callus induction from the peduncles and 2, 4 — D. is better for callus induction from the fruit skins. 10mgL-1 NAA + 0.5mgL-1 BAP are best hormone combination for earlier callus formation and to obtain higher volume callus from grape peduncles.
Callus induction of grapes using peduncles and fruit *skins can be accomplished successfully using above methods of surface sterilization and culture establishment can be used successfully.
Key words: Callus induction, Peduncles, Fruit Skins, NAA, BAP, KIN, 2, 4-D
Description
Keywords
Export Agriculture Degree Programme (EAG)