Browsing by Author "Somarathna, K.M.D.M."
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Item Determination of the Appropriate ayza sativa (Rice) Straw Fertilizer Loading Level to Enhance Survival Rate of Catla calla (Catla) Post Larvae(Uva Wellassa University of Sri Lanka, 2018) Maddumaarachchi, M.A.D.U.; Somarathna, K.M.D.M.; Liyanage, N.P.P.Introduction of the Indian major carp species like Catla calla (Catla), Labeo rohita (Rohu) and Cirrhinus mrigala (Mirigal) has been resulted in a significant development in inland fisheries in Sri Lanka. Among these introduced fish species, Catla contributes to freshwater fish production in Sri Lanka significantly. One of the identified problems in Aquaculture Development Center, Udawalawa was lower survival rate of Catla post larvae at nursery stage. Hence this study was conducted to determine appropriate rice straw loading level to enhance survival rate of Catla post larvae reared in nursery tanks of 20 m2. The experiment was conducted using a Completely Randomized Design (CRD). A preliminary fertilizing procedure was done to determine a defenitive range (75-90 g m2 of dried rice straw) to conduct the experiment and the tanks were fertilized at above rates. Body weights and total lengths of post larvae were recorded at stocking and water quality parameters were recorded twice a day in each treatment. Number of survived post larvae in each treatment were recorded with the body weights and total lengths at the end of the rearing period. Recorded data were analyzed using one way ANOVA and Tukey test was used to compare mean values at 5% significance level. Total plankton count, survival rate, length gain and weight gain of post larvae were significantly different (p<0.05) among treatments. 80 g m-2 loading level of rice straw was the best treatment showing highest survival rate (66.6210.69), greatest length gain (159.0816.99 cm), maximum weight gain (2,5761176g) and highest mean value of total plankton count (54,9101646). Adequate amounts of planktons and proper water quality parameters act as major reasons for higher survival rate of Catla post larvae. Therefore, the loading rate of 80 g In-2 was found to be best among treatments for fertilizing Catla nursery tanks with rice straw to enhance survival rate.Item A preliminary study on the effectiveness of organic fertilizers for the survival rate of Catla catla(Uva Wellassa University of Sri Lanka, 2015) Prabhani, K.G.M.; Liyanage, N.P.P.; Somarathna, K.M.D.M.Fish can be considered as one of the main sources of animal protein (26.2%) in human diet (Khan et al., 2004). Fish also become a popular source of food in Asia and developing countries. Both aquaculture and fisheries activities conducting in marine and inland waters, cater the protein requirement of people in Sri Lanka. Indian and Chinese Carp species are commonly used today in inland aquaculture. Catla catla (Catla) is a zooplankton feeding Indian major carp species which was introduced to reservoirs in Sri Lanka to uplift the inland fishery production. This species has become a very popular fish species among the fish farmers due to higher growth rate, ability to co-exist with other major carps and consumer preference. Aquaculture Development Center (AQDC), Udawalawa belongs to National Aquaculture Development Authority (NAQDA) is producing fish seeds of carp species including Catla, for the development of inland aquaculture and fisheries. The problem identified in fish seed production in the AQDCs was the low survival rate of Catla at rearing of post larvae (PL) to fry stage. Moreover, it was observed that survival rate in mud ponds is lower than the survival rates in cement tanks. In the AQDC Udawalawa, cow dung is commonly used for fertilization of rearing tanks and ponds. This study was carried out to find out what the best effective organic fertilizer for the higher survival rate and growth of Catla catla. Cow dung, Leucaena leucocephala (Ipilipil) and Oriza sativa (rice) straw were selected as organic fertilizers as they are of low cost and readily available in the area of the study. Materials and Methods The experiment was conducted in nursery ponds (mud ponds) and nursery cement tanks in AQDC, Udawalawa. A mud pond and a cement tank which were already fertilized during five days (as normal practice in the AQDC) using cow dung (250g/m ) were selected and plankton densities (individuals per liter) were recorded by assuming ponds were reached optimum plankton density. Mean value of those densities was calculated and used as baseline value. Dried cow dung (250g/m ) was selected as control for the study as it was the normal practice in the AQDC. Rice straw (30g/m ) and dried Ipilipil (30g/m ) were the selected treatments for the study. These are the commonly used fertilizer concentrations in the field. Initially, the time taken to reach the plankton density to the baseline value was recorded for Rice straw and Ipilipil fertilizer types. The surrounding of three mud ponds was cleaned and four hapa net cages were fixed to the bottom of the three mud ponds to prepare four replicates. All the four replicates were fixed in same way to maintain the same volume of water. Firstly the ponds were filled up to 50 cm with water. Complete Randomized Design (CRD) was conducted as the experimental design. Four days old PL in a single cohort, who spent seven days in hatchery were stocked in the morning in cement tanks and hapa net cages in mud ponds. The recommended stocking density in tanks and ponds was 250-300 PLs per square meter (FAO, 2014). Three hundred PL were stocked in cement tanks per square meter and 250 PL per square meter were stocked in hapa net cages in mud ponds. Altogether 54 000 PL were stocked in nine cement tanks including 6 000 PL in each tank (20 m ) and 6 000 PL were stocked in 12 hapa net cages fixed in three mud ponds including 500 PL in each cage (2 m ). Harvesting of fry (2.5 cm) was done after three weeks rearing time period. Total length (TL) and weight were measured weekly using randomly taken samples (10% of population) of PLs from each tank and happa, until harvesting. Analytical balance (±0.001 g) was used in measuring the weights of PL. Venire caliper (±0.01 cm) was used for measuring the total length of PL. Average weight and average TL was calculated using the data obtained. Temperature and pH were measured daily using electronic pH meter and thermometer. Dissolved oxygen (DO) level was observed using Winkler method to keep the treatment units at the optimum DO level during the study period. Secchi disk values were taken weekly by using secchi disk. Percentage survival rate, percentage weight gain (PWG) and percentage length gain (PLG) were calculated (Okunsebor and Ayuma, 2011). Obtained data were analyzed using analysis of variance (ANOVA) using the General Linear Model (GLM) procedure of MINITAB 16 and Excel 2010 office package. Results The highest value of survival rate could be observed in the treatment of rice straw as 80.02 % (< P = 0.05) in cement tanks. The highest value of survival rate in mud ponds could be observed in the treatment of rice straw as 36.45 % (< P = 0.05). The highest percentage length gain (PLG) (309.29±2.50 cm) and the highest percentage weight (PWG) gain (4667±252 g) were observed in the treatment of rice straw in cement tanks (P <0.05). Further the highest PWG (14950±173) and the highest PLG (305.73±2.60) were observed in the mud ponds fertilized by rice straw (P < 0.05). In considering the values of plankton count, the highest mean value of phytoplankton number in cement tanks could be observed in the ponds fertilized by cow dung (control). The lowest mean value was observed in the ponds fertilized by rice straw. In mud ponds also the same results were obtained at the date of stocking PL for rearing up to fry. The highest zooplankton count in cement tanks could be observed in the ponds fertilized by rice straw while the lowest value was observed in the ponds fertilized by rice straw. In mud ponds also the highest zooplankton count was observed in the treatment of rice straw at the date of stocking PL. As Catla catla was a zooplankton feeder the highest survival rate, the mean values of highest PWG and highest PLG were observed in the treatment unit of rice straw that included highest zooplankton amount in the water. The highest zooplankton amount in the water could provide more live feed and it might be the reason for the highest survival and growth of fry at harvesting. The highest secchi disk value was observed in the treatment of rice straw throughout the study period in both cement tanks and mud ponds. Discussion European Inland Fisheries Advisory Commission indicates that turbidity can have a harmful impact on freshwater fish by reducing food supplies and affecting gill function, killing them or reducing their growth rate, preventing successful development of fish egg and larvae and affecting the efficiency of methods for catching fish (MPCA, 2008). Very high phytoplankton blooms create high turbidity and it causes negative effects on survival of larvae. In this study also shows that both cement tanks and mud ponds fertilized by rice straw shows lower phytoplankton and higher zooplankton community. Fluten et al. 2002 found that feeding and growth rates reduced due to more turbid water. The lower turbidity could be observed in the treatment of rice straw than two other treatments. Catla PL can easily find out their live feed in low turbid water. It was commonly found copepods, daphnia and rotifers as zooplankton in water sampled in the mud ponds and cement tanks fertilized by rice straw.