A preliminary study on the effectiveness of organic fertilizers for the survival rate of Catla catla
No Thumbnail Available
Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Uva Wellassa University of Sri Lanka
Abstract
Fish can be considered as one of the main sources of animal protein (26.2%) in human diet (Khan et
al., 2004). Fish also become a popular source of food in Asia and developing countries. Both
aquaculture and fisheries activities conducting in marine and inland waters, cater the protein
requirement of people in Sri Lanka. Indian and Chinese Carp species are commonly used today in
inland aquaculture. Catla catla (Catla) is a zooplankton feeding Indian major carp species which was
introduced to reservoirs in Sri Lanka to uplift the inland fishery production. This species has become
a very popular fish species among the fish farmers due to higher growth rate, ability to co-exist with
other major carps and consumer preference. Aquaculture Development Center (AQDC), Udawalawa
belongs to National Aquaculture Development Authority (NAQDA) is producing fish seeds of carp
species including Catla, for the development of inland aquaculture and fisheries. The problem
identified in fish seed production in the AQDCs was the low survival rate of Catla at rearing of post
larvae (PL) to fry stage. Moreover, it was observed that survival rate in mud ponds is lower than the
survival rates in cement tanks. In the AQDC Udawalawa, cow dung is commonly used for
fertilization of rearing tanks and ponds. This study was carried out to find out what the best effective
organic fertilizer for the higher survival rate and growth of Catla catla. Cow dung, Leucaena
leucocephala (Ipilipil) and Oriza sativa (rice) straw were selected as organic fertilizers as they are of
low cost and readily available in the area of the study.
Materials and Methods
The experiment was conducted in nursery ponds (mud ponds) and nursery cement tanks in AQDC,
Udawalawa. A mud pond and a cement tank which were already fertilized during five days (as normal
practice in the AQDC) using cow dung (250g/m ) were selected and plankton densities (individuals
per liter) were recorded by assuming ponds were reached optimum plankton density. Mean value of
those densities was calculated and used as baseline value. Dried cow dung (250g/m ) was selected as
control for the study as it was the normal practice in the AQDC. Rice straw (30g/m ) and dried Ipilipil
(30g/m ) were the selected treatments for the study. These are the commonly used fertilizer
concentrations in the field. Initially, the time taken to reach the plankton density to the baseline value
was recorded for Rice straw and Ipilipil fertilizer types.
The surrounding of three mud ponds was cleaned and four hapa net cages were fixed to the bottom
of the three mud ponds to prepare four replicates. All the four replicates were fixed in same way to
maintain the same volume of water. Firstly the ponds were filled up to 50 cm with water. Complete
Randomized Design (CRD) was conducted as the experimental design.
Four days old PL in a single cohort, who spent seven days in hatchery were stocked in the morning
in cement tanks and hapa net cages in mud ponds. The recommended stocking density in tanks and
ponds was 250-300 PLs per square meter (FAO, 2014). Three hundred PL were stocked in cement
tanks per square meter and 250 PL per square meter were stocked in hapa net cages in mud ponds.
Altogether 54 000 PL were stocked in nine cement tanks including 6 000 PL in each tank (20 m )
and 6 000 PL were stocked in 12 hapa net cages fixed in three mud ponds including 500 PL in each
cage (2 m ). Harvesting of fry (2.5 cm) was done after three weeks rearing time period. Total length
(TL) and weight were measured weekly using randomly taken samples (10% of population) of PLs
from each tank and happa, until harvesting. Analytical balance (±0.001 g) was used in measuring the
weights of PL. Venire caliper (±0.01 cm) was used for measuring the total length of PL. Average
weight and average TL was calculated using the data obtained.
Temperature and pH were measured daily using electronic pH meter and thermometer. Dissolved
oxygen (DO) level was observed using Winkler method to keep the treatment units at the optimum
DO level during the study period. Secchi disk values were taken weekly by using secchi disk.
Percentage survival rate, percentage weight gain (PWG) and percentage length gain (PLG) were
calculated (Okunsebor and Ayuma, 2011). Obtained data were analyzed using analysis of variance
(ANOVA) using the General Linear Model (GLM) procedure of MINITAB 16 and Excel 2010 office
package.
Results
The highest value of survival rate could be observed in the treatment of rice straw as 80.02 % (< P =
0.05) in cement tanks. The highest value of survival rate in mud ponds could be observed in the
treatment of rice straw as 36.45 % (< P = 0.05). The highest percentage length gain (PLG)
(309.29±2.50 cm) and the highest percentage weight (PWG) gain (4667±252 g) were observed in the
treatment of rice straw in cement tanks (P <0.05). Further the highest PWG (14950±173) and the
highest PLG (305.73±2.60) were observed in the mud ponds fertilized by rice straw (P < 0.05). In
considering the values of plankton count, the highest mean value of phytoplankton number in cement
tanks could be observed in the ponds fertilized by cow dung (control). The lowest mean value was
observed in the ponds fertilized by rice straw. In mud ponds also the same results were obtained at
the date of stocking PL for rearing up to fry. The highest zooplankton count in cement tanks could
be observed in the ponds fertilized by rice straw while the lowest value was observed in the ponds
fertilized by rice straw. In mud ponds also the highest zooplankton count was observed in the
treatment of rice straw at the date of stocking PL. As Catla catla was a zooplankton feeder the highest
survival rate, the mean values of highest PWG and highest PLG were observed in the treatment unit
of rice straw that included highest zooplankton amount in the water. The highest zooplankton amount
in the water could provide more live feed and it might be the reason for the highest survival and
growth of fry at harvesting. The highest secchi disk value was observed in the treatment of rice straw
throughout the study period in both cement tanks and mud ponds.
Discussion
European Inland Fisheries Advisory Commission indicates that turbidity can have a harmful impact
on freshwater fish by reducing food supplies and affecting gill function, killing them or reducing their
growth rate, preventing successful development of fish egg and larvae and affecting the efficiency of
methods for catching fish (MPCA, 2008). Very high phytoplankton blooms create high turbidity and
it causes negative effects on survival of larvae. In this study also shows that both cement tanks and
mud ponds fertilized by rice straw shows lower phytoplankton and higher zooplankton community.
Fluten et al. 2002 found that feeding and growth rates reduced due to more turbid water. The lower
turbidity could be observed in the treatment of rice straw than two other treatments. Catla PL can
easily find out their live feed in low turbid water. It was commonly found copepods, daphnia and
rotifers as zooplankton in water sampled in the mud ponds and cement tanks fertilized by rice straw.
Description
Keywords
organic Fertilizers, Agriculture, Export Agriculture, Animal Sciences, Aquaculture and Fisheries, Food Science