Browsing by Author "Meepegamage, L.S."

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Development of Bioactive Peptides from Hilsa Kelee Fish Crude Protein and Checking the Functional Properties of its Hydrolysates
    (Uva Wellassa University of Sri Lanka, 2016) Meepegamage, L.S.
    Hilsa kelee also known as "Karattaya" is a marine pelagic, seasonal fish species which has a high catch during April to September. It mainly used to produce fish meal and baits even though it has high nutrients. Nevertheless, it has a low market value and demand due to its bony structure leading to high wastage. Production of fish Protein Hydrolysates (FPH) can be an alternative. Therefore, the study was designed to determine the functional properties of FPH produced from, Hilsa kelee fish crude protein (water and salt) and determine it's the functional properties of its hydrolysates. Crude protein 20mg/m1 concentrated extractions were mixed with Protease from Bacillus licheniformis (60°C, pH 6.5-8.5), Papain from papaya latex (65°C, pH 5.8-7.0), a-chymotrypsin from bovine pancreas (50°C, pH 7.8) and Elastase from porcine pancreas (37°C, pH 7.8-8.5) separately in 1:100 ratios (enzyme: substrate =1:100). Each sample was incubated under different time period (0, 3, 6, 9, 12 and 24hrs) in their optimum temperatures. According to 15% SDS-PAGE analysis Protease — 3 h for both water and salt extraction, Papain — 6 h for water extraction and 3h for salt extracted FPC, a-chymotrypsin — 6h for both water and salt extraction and Elastase — 6h for both water and salt extraction was determined as the best time combinations. TBARS assay, Ferrous chelation activity and antibacterial activities by agar well diffusion method. All treatments were triplicated (n=3). According to the results obtained from TBARS assay, FPHs from Papain showed highest antioxidant properties (5.84±0.05 MDA mg/1 in water extraction) (p>0.05). Fe (II) chelation activity analysis revealed the FPHs produced from a-chymotrypsin showed the highest chelation (20.81±0.02% in water extraction) (p<0.05). All hydrolysates did not show any antimicrobial activities (p>0.05). Thus, FPHs produced using a-chymotrypsin-3 h contain strong antioxidant activity while others did not contain antimicrobial activity nor iron chelating properties. Keywords: Hilsa kelee, Fish Protein Hydrolysates (FPH), Antioxidant, Metal Chelating, Antibacterial activity
  • No Thumbnail Available
    Item
    Functional Properties of Hydrolysates of Bioactive Peptides Extracted from Water Soluble Crude Protein from Hilsa kelee
    (Uva Wellassa University of Sri Lanka, 2021) Meepegamage, L.S.; Abeyrathne, E.D.N.S.
    Hilsa kelee also known as “Karattaya” is a marine pelagic, seasonal fish species which has a high catch during April to September. It is mainly used to produce fish meal and used as baits even though it has high nutrients. In addition, it has a low market value and a demand due to its bony structure leading to high wastage and production of fish protein hydrolysates (FPH) can be an alternative. 20 mg/ml concentrated water-soluble crude protein extractions were mixed with Protease from Bacillus licheniformis (60°C, pH 6.5-8.5), Papain from papaya latex (65°C, pH 5.8- 7.0), α-chymotrypsin from bovine pancreas (50°C, pH 7.8) and Elastase from porcine pancreas (37°C, pH 7.8-8.5) separately in 1:100 (enzyme: substrate) ratios. Each sample was incubated at different time intervals (0, 3, 6, 9, 12 and 24 hr) in predetermined optimum pH and temperatures. Then, 15% SDS-PAGE was used to determine the best hydrolysis condition. Bulk hydrolysates were prepared with Papain-6 h, Protease-3 h, α-chymotrypsin-3 h, Elastase-6 h followed with heat inactivation at 100°C for 15 miutes for further experiments. Then, TBARS assay, Ferrous chelation activity and antibacterial activities by agar well diffusion method was conducted. All treatments were triplicated (n=3). According to the results obtained from TBARS assay, FPHs from papain showed the highest antioxidant properties (5.84±0.05 MDA mg/L) compared to other treatments (p>0.05). Fe (II) chelation activity analysis revealed the FPHs produced from α-chymotrypsin showed the highest chelation (20.81±0.02%) (p<0.05). None of the hydrolysates showed any antimicrobial activities for tested microorganismas up to 20,000 ppm. Thus, FPHs produced using papain for 6 h followed with heat inactivation at 100°C for 15 miutes contain strong antioxidant activity Keywords: Hilsa kelee; Fish Protein Hydrolysates (FPH); Antioxidant; Metal Chelating; Antibacterial activity