Browsing by Author "Herath, H.M.I."

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    Development of an Appropriate Micro Propagation Protocol for Nymphaea×erangae
    (Uva Wellassa University of Sri Lanka, 2021) Wijekoon, W.M.A.P.; Herath, H.M.I.; Bambaranda, B.V.A.S.M.
    The Nymphaea × erangae Yakandawala, Guruge & Yakandawala is one of the most conspicuously attractive and eye catching plant which has high demand in local and export markets. However the production of these plants takes a long time where wild collection is not sustainable and cannot cater the demand. Micro propagation is one of the best techniques which can be practiced in commercial production of Nymphaea × erangae plants. The studies on Nymphaea × erangae are very limited and the contamination rates is very high when the mother plants collected from muddy soil media,can easily exposed and contaminated by microbes. This research study focuses to investigate the ability to use soil less culture system for mother plants of Nymphaea × erangae to minimize contamination in micro propagation. Three treatments ( duration of 1, 2, and 4 weeks maintenance of mother plants in soil less culture system treated by 0.5g/1L Albert solution and 1g/10L Fungicide in twice a week) were tested and plants were maintained in muddy soil were used as the control. The leaf blade and rhizome buds were used as explants. The results revealed Nymphaea × erangae tissue culture in MS media, which were placed for a week in the soil less culture system showed significantly higher (p<0.05) survival rate and 25 % of non-contaminated surviving culture vessels after two months, compared to control and other treatments, which is suitable for micropropagation of Nymphaea × erangae in commercial scale. Keywords: Nymphaea × erangae Yakandawala, Guruge & Yakandawala, Soil less culture system, MS medium, Albert solution, Fungicide
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    Development of micropropagation protocols for two different Cryptocoryne species in Sri Lanka (Cryptocoryne parva and Cryptocoryne xwillisii)
    (Uva Wellassa University of Sri Lanka, 2016) Boyagoda, D.T.K.; Jayamanne, S.C.; Herath, H.M.I.
    An efficient protocol was developed for the micropropagation of Cryptocoryne parva and Cryptocoryne xwillisiii, two endemic threatened aquatic herbs of Sri Lanka, which considered as highly valued aquarium plants. Rhizomes and leaves of two species were surface sterilized using Sodium Hypochlorite and 0.1 % Mercuric Chloride successfully and they were established on full strength Murashige and Skoog (MS) medium. Experiment was arranged in factorial Complete Randomized Design (CRD). Effects of basal MS medium supplementation with factorial combinations of BAP 6- benzylaminopurine (0, 1, 3, 5mg r') and NAA-Naphthalene Acetic Acid (0, 0.1, 0.3 mg 1') on shoot initiation from rhizome and leaf explants were determined after 42 days. Data were analyzed using ANOVA and Tukey's Test incorporated in MINITAB 16 software. The presence of growth regulators in MS medium had a significant (p<0.05) effect on shoot initiation in rhizomes of both species. Highest mean number of shoots initiated per single rhizome explant was observed in the combination of 5.0 mg 1-' BAPwith 0.1 mg V NAA in both species (C. parva 4.3, 3.7; C. xwillisii 4.1). Regenerated shoots of rhizome explants were cultured on MS medium supplemented with BAP 0, 1, 3 mg r' for shoot multiplication. The presence of growth regulators in MS medium had a highly significant (p<0.05) effect on shoot proliferation of both species. Highest mean number of shoots proliferated per shoot (C. parva? and C. xwillisii 5.6) was observed in 3 mg r' of BAP. Presence of growth regulators in MS medium had a highly significant (P<0.05) effect on embossing leaves of both species as a positive response. Best explant source for both species was rhizome. Optimum hormone combination for shoot initiation of both species is 5 mg of BAP with 0.1 mg r' of NAA and for shoot multiplication optimum hormone concentration is 3 mg 1-1ofBAP. Keywords: Cryptocoryne parva, Cryptocoryne xwillisii, BAP, NAA, Rhizomes and leaves, Shoot initiation, Shoot multiplication
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    Effect of Sargassum wightii (Seaweed) Extract on In-vitro Sub-Culture Medium of Dendrobium Orchid
    (Uva Wellassa University of Sri Lanka, 2019) Madubhashi, R.M.M.B.; Herath, H.M.I.; Kaliyadasa, P.E.; Perera, G.Y.A.D.D.
    Dendrobium is one of the most beautiful orchid varieties having high demand as a cut flower as well as a potted plant. Large scale commercial production is possible only through vegetative propagation such as tissue culture. High cost of production is a major drawback of tissue culture which involves higher expenses for chemicals. This study focused on identification of bio-stimulatory effects of a seaweed (Sargassum wightii) extract on in-vitro sub culture medium of Dendrobium for quality plantlet production. Two experiments were carried out with 20 treatments in 10 replicates where, MS (Murashige and Skoog) basal medium with BAP (6-Benzyl Amino Purine) and without BAP were used for experiment one. For experiment two KnC (Knudson C) basal medium was used with coconut water, banana pulp and without coconut water, banana pulp. Treatments were prepared with combination of seaweed extract of 5%, 10%, 25% and 40% concentrations for above basal media. MS medium with BAP and KnC medium with coconut water, banana pulp without adding seaweed extract were used as control. Plantlets in MS medium with BAP and 10% seaweed extract showed significantly higher (p<0.05) plant height, number of shoots, number of leaves and dark green color leaves whereas higher number of roots were recorded in MS medium without BAP but with 10% seaweed extract compared to the control. Plantlets in KnC medium supplemented with coconut water, banana pulp and 10% seaweed extract showed significantly higher (p<0.05) plant height, number of shoots, number of leaves, number of roots and dark green color leaves compared to the control. Comparatively, MS medium with BAP and 10% seaweed extract showed good performance, which is suitable for commercial plant production.