Development of an Appropriate Micro Propagation Protocol for Nymphaea×erangae
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Uva Wellassa University of Sri Lanka
Abstract
The Nymphaea × erangae Yakandawala, Guruge & Yakandawala is one of the most conspicuously
attractive and eye catching plant which has high demand in local and export markets. However the
production of these plants takes a long time where wild collection is not sustainable and cannot cater
the demand. Micro propagation is one of the best techniques which can be practiced in commercial
production of Nymphaea × erangae plants. The studies on Nymphaea × erangae are very limited and
the contamination rates is very high when the mother plants collected from muddy soil media,can
easily exposed and contaminated by microbes. This research study focuses to investigate the ability to
use soil less culture system for mother plants of Nymphaea × erangae to minimize contamination in
micro propagation. Three treatments ( duration of 1, 2, and 4 weeks maintenance of mother plants in
soil less culture system treated by 0.5g/1L Albert solution and 1g/10L Fungicide in twice a week)
were tested and plants were maintained in muddy soil were used as the control. The leaf blade and
rhizome buds were used as explants. The results revealed Nymphaea × erangae tissue culture in MS
media, which were placed for a week in the soil less culture system showed significantly higher
(p<0.05) survival rate and 25 % of non-contaminated surviving culture vessels after two months,
compared to control and other treatments, which is suitable for micropropagation of Nymphaea ×
erangae in commercial scale.
Keywords: Nymphaea × erangae Yakandawala, Guruge & Yakandawala, Soil less culture system,
MS medium, Albert solution, Fungicide
Description
Keywords
Agriculture, Soil Science, Animal Sciences, Weed