Browsing by Author "Herath, H.M.D.M.P."

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    Initiation of Callogenesis from Unfertilized Ovary Explants of Oil Palm (Elaeis guineensis Jacq.)
    (Uva Wellassa University of Sri Lanka, 2013) Herath, H.M.D.M.P.; Alwis, L.M.H.R; Bandhupriya, H.D.D.
    Oil palm is an efficient oil producing crop which has high economic value. Due to less capability of vegetative propagation, tissue culture is considered to be the only approach to produce clonal plantlets for oil palm. Several research based on somatic embryogenesis have been undertaken using various tissues (Euewens, 1976; Guedes et al., 2011) but no research work on oil palm ovary explants is reported. The objectives of this study were to find out relationship between maturity stages of inflorescence for callogenesis, to identify suitable in vitro culture media for callogenesis, and to determine the optimum 2, 4-D concentration for callus induction and multiplication in same callusing media. Methodology Unfertilized ovaries obtained from immature female inflorescences of oil palm (Elaeis guineensisJacq.) were tested as a source of explants for callogenesis. Inflorescence maturity stages of -2, -3 and -4 were cultured on Y3 (Eeuwens, 1976), CRI-72 (Karunarathne and Periypperuma, 1989) and OPC3 medium (Karun and Sajini, 1996) supplemented with 2,4-D levels of 160 µM, 180 µM and 200 µM. Collected female flowers were disinfected wit h 5% commercial bleach (chlorox) for 10 minutes followed by five rinses with sterile water. Ovaries were dissected out and cultured in vials containing 10 ml of callus induction medium under C in an incubation room until callus proliferation. Cultured ovaries were sub-cultured in to fresh medium (same callusing medium) and callus multiplication possibility was tested. Complete Randomized Design (CRD) with three factor factorial analysis was used as the experimental design.
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    Initiation of Callogenesis from Unfertilized Ovary Explants of Oil Palm (Elaes Guineensis Jacq)
    (Uva Wellassa University of Sri Lanka, 2013) Herath, H.M.D.M.P.
    Oil palm is an efficient oil producing crop which has high economic value. Due to lack of vegetative propagation method, tissue culture remains the only approach to produce clonal plantlets for oil palm. Unfertilized ovaries obtained from immature female inflorescences of oil palm (Elaeis guineensis Jacq.) were tested as a source of explants for callogenesis. The callus induction possibility was examined through maturity stages of inflorescence, different culture media and 2,4-D concentrations. Callus multiplication possibility using the same callusing media was tested. Inflorescence maturity stages of -2, -3 and -4 were cultured on Y3, CRI 72 and OPC3 media supplemented with 2,4-D levels of 160 pM, 180 IAM and 200 p,M. Development of callus was visible during the seven weeks after culture. Callus proliferation occurred when subcultured in to the same medium. It was found that there was no significant difference and interactive effect among treatments tested. The results achieved suggest that unfertilized ovaries of immature female flowers of oil palm (Elaeis guineensis Jacq.) can be used as alternative sources of explants for the induction of callogenesis. Callus tissues of oil palm ovaries can be established and grown in culture. Callus can be subcultured in the same callusing medium and it is possible to multiply the callus. Key Words: Oil palm, Callogenesis, Ovary explants, in vitro culture, Culture medium