Browsing by Author "Gunawardane, M.H.M.A.S.V."

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    Pseudomonas fluorescens BG-E, a Potential Biological Control Agent for Bloom- forming Cyanobacterial genus, Pseudanabaena
    (Uva Wellassa University of Sri Lanka, 2021) Wijesooriya, M.M.; Gunawardane, M.H.M.A.S.V.; Randima, G.W.A.P.; Gamage, S.M.K.W.; Masakorala, K.
    Outbreaks of toxin-producing cynobacterial blooms in freshwater reservoirs in Sri Lanka have increased over the past few decades and are likely to be responsible for fish death reported in many reserviores. Various physical and chemical methods are in practice to eliminate cyanobacterial blooms. However, due to some limitaions adhered with those practices, currently researchers have focused on the applicability of algicidal bacteria as an environmental friendly sustainable control strategy. The present study aimed to investigate cyanolytic and microcystin-LR (MC-LR), degrading potential of heterotrophic bacteria isolated from freshwaters. Sub-surface water samples were collected from Bandagiriya reservoir in Hambantota district. Bacteria were isolated from a composite water sample collected from 22 sampling sites representing the entire reservoir in 50% nutrient agar. Axenic cultures of colonial (Microcystis sp., Synechoccus sp.) and filamentous (Pseudanabaena sp., Pseudanabaena lonchoids, Leptolyngbya, and Geitlerinema sp.) cyanobacterial genera were used as tested cyanobacteria. Seven morphologically distinct bacterial isolates were screened for the lytic activity against cyanobacteria in BG11 broth. Briefly, cyanobacterial cultures at ~ 2×106 cells/mL were inoculated with 10% v/v each bacterial isoates at ~ 1×108 cells/mL. Following 10 days of incubation, distinct discoloration of blue-green into yellowish-brown color in the cell mass of two species of Pseudanabaena was observed in cultures inoculated with BG-E bacterial isolate. Microscopic images provided evidence for complete disintegration of filamentous structures. Disappearance of blue-green color might be due to the oxidation of released photosynthetic pigments during cell wall disintegration. The % lytic activity of BG-E against Pseudanabaena sp. and Pseudanabaena lonchoids based on the chlorophyll-a analysis were 82% and 73% respectively. Bacterial isolate BG-E was identified as Pseudomonas fluorescens by sequencing of its 16S rRNA gene. Since Pseudanabaena is a MC-LR producing and frequently found filamentous form in freshwater reservoirs of Sri Lanka, MC-LR biodegradation potential of BG-E was investigated. Results showed that BG-E is not capable of degrading MC-LR at tested concentrations. Further, none of the genes in the microcystin-degrading gene cluster, mlrABCD were amplified in polymerase chain reaction and might be the reason for the incapability in degrading MC-LR. However, strong cyanolytic activity highlights potential application of P. fluorescens BG-E in future biological control strategies in Sri Lanka. Keywords: Cell lysis; Cyanobacteria; Cyanotoxin; Pseudanabaena; Pseudomonas fluorescens
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    Trophic Status and Spatial Variation of Cyanobacterial Diversity in Lunugamwehera Reservoir, Sri Lanka
    (Uva Wellassa University of Sri Lanka, 2020) Gunawardane, M.H.M.A.S.V.; Gamage, S.M.K. Widana; Wijesooriya, M.M.; Randima, G.W.A.P.; Masakorala, K.; Atapattu, K.S.S.; Sanjaya, K.
    Lunugamwehera is a large man-made reservoir located in the South Eastern dry zone of Sri Lanka. It is the main source of drinking water, irrigation, and fisheries for more than 5000 families in the area. The occurrence of cyanobacterial blooms or high population densities in water bodies results in various environmental and health issues. Population density and composition of cyanobacteria in a waterbody may vary with the trophic status of water. There is a high potential of contaminating the Lunugamwehera reservoir with mineral nutrients that come from agricultural lands. However, considerable attention has not been paid to study trophic status and cyanobacterial diversity of the Lunugamwehera reservoir. Therefore, our objective was to determine the spatial variation of cyanobacterial diversity in the Lunugamwehera reservoir along with trophic status. Sub-surface water samples collected from 28 sampling sites representing the whole waterbody were microscopically examined to identify cyanobacterial species. Total phosphorous concentration, total chlorophyll-a concentration, and Secchi depth were determined to assess trophic status using Carlson Trophic State Index. Also, water quality parameters were measured using a multiparameter water quality meter. Microscopic analysis revealed the presence of only two cyanobacterial species, Microcystis and Oscillatoria implying less cyanobacterial diversity in the reservoir. Microcystis spp. (~3.257 x 107 cells mL-1 ) dominated all sampling sites with significant difference (p<0.05) in cell densities among sites while Oscillatoria spp. (5 - 10 cells mL-1 ) occurred rarely. Apart from cyanobacteria, Pediastrum spp., Tribonema spp. and Nitzschia spp. (1 - 2 cells mL-1 ) were also identified scarcely. Water quality parameters were approximately similar in all sampling sites except increased turbidity near the inlet. According to the Carlson Trophic State Index (37.70), the water body was ‘slightly oligotrophic’. Therefore, the overall results infer the presence of less cyanobacterial diversity and significant spatial variation in Microcystis spp. as the dominant species in the slightly oligotrophic water of the Lunugamwehera reservoir. Keywords: Carlson trophic state index, Cyanobacteria, Oligotrophic, Spatial variation