Pseudomonas fluorescens BG-E, a Potential Biological Control Agent for Bloom- forming Cyanobacterial genus, Pseudanabaena
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Date
2021
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Uva Wellassa University of Sri Lanka
Abstract
Outbreaks of toxin-producing cynobacterial blooms in freshwater reservoirs in Sri Lanka have
increased over the past few decades and are likely to be responsible for fish death reported in many
reserviores. Various physical and chemical methods are in practice to eliminate cyanobacterial
blooms. However, due to some limitaions adhered with those practices, currently researchers have
focused on the applicability of algicidal bacteria as an environmental friendly sustainable control
strategy. The present study aimed to investigate cyanolytic and microcystin-LR (MC-LR), degrading
potential of heterotrophic bacteria isolated from freshwaters. Sub-surface water samples were
collected from Bandagiriya reservoir in Hambantota district. Bacteria were isolated from a composite
water sample collected from 22 sampling sites representing the entire reservoir in 50% nutrient agar.
Axenic cultures of colonial (Microcystis sp., Synechoccus sp.) and filamentous (Pseudanabaena sp.,
Pseudanabaena lonchoids, Leptolyngbya, and Geitlerinema sp.) cyanobacterial genera were used as
tested cyanobacteria. Seven morphologically distinct bacterial isolates were screened for the lytic
activity against cyanobacteria in BG11 broth. Briefly, cyanobacterial cultures at ~ 2×106 cells/mL
were inoculated with 10% v/v each bacterial isoates at ~ 1×108 cells/mL. Following 10 days of
incubation, distinct discoloration of blue-green into yellowish-brown color in the cell mass of two
species of Pseudanabaena was observed in cultures inoculated with BG-E bacterial isolate.
Microscopic images provided evidence for complete disintegration of filamentous structures.
Disappearance of blue-green color might be due to the oxidation of released photosynthetic pigments
during cell wall disintegration. The % lytic activity of BG-E against Pseudanabaena sp. and
Pseudanabaena lonchoids based on the chlorophyll-a analysis were 82% and 73% respectively.
Bacterial isolate BG-E was identified as Pseudomonas fluorescens by sequencing of its 16S rRNA
gene. Since Pseudanabaena is a MC-LR producing and frequently found filamentous form in
freshwater reservoirs of Sri Lanka, MC-LR biodegradation potential of BG-E was investigated.
Results showed that BG-E is not capable of degrading MC-LR at tested concentrations. Further, none
of the genes in the microcystin-degrading gene cluster, mlrABCD were amplified in polymerase
chain reaction and might be the reason for the incapability in degrading MC-LR. However, strong
cyanolytic activity highlights potential application of P. fluorescens BG-E in future biological control
strategies in Sri Lanka.
Keywords: Cell lysis; Cyanobacteria; Cyanotoxin; Pseudanabaena; Pseudomonas fluorescens
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Keywords
Environment Science, Botany, Flowers Cultivation, Biological Control