Induruwage, S.S.Alakolanga, A.G.A.W.Rathnayake, S.I.Senarathna, K.G.C.Wijesekara, K.B.2021-02-192021-02-1920209789550481293http://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/6071/proceeding_oct_08-433.pdf?sequence=1&isAllowed=yDiabetes mellitus is one of the non-contagious diseases which has now become a major health problem all over the world. This study was conducted to identify a natural, safe, and reliable solution for “diabetes” with proper scientific validation. The objective of the study was to extract and isolate anti-hyperglycemic compounds from Cheilocostus speciosus leaves. For this, a successive extraction with diethyl ether, ethyl acetate, and ethanol using sonication at room temperature was performed to extract oven-dried (40 ℃, 48 h) leaves of C. speciosus. Percentage inhibition of the enzymes and the IC50 values were determined. But the extracts of diethyl ether and ethyl acetate did not exhibit proper percent inhibitions for both alpha-amylase and alpha-glucosidase activities. Porcine pancreatic alpha-amylase inhibitory activity was performed using the DNSA method and it was detected in ethanolic extract of C. speciosus leaves with an IC50 of 17.12 mg ml-1 and Saccharomyces cerevisiae alpha-glucosidase inhibitory activity was detected with an IC50 of 21.20 mg ml-1 . Both enzyme inhibitory activities were compared with that of commercial Acarbose (IC50 values for alpha-amylase and alpha-glucosidase enzymes are 65.44 µg ml-1 and 68.52 µg ml-1 , respectively). When comparing with acarbose, a crude extract of C. speciosus leaves show promising nature to isolate antihyperglycemic compounds. Results also indicate that the alpha-amylase and alpha-glucosidase inhibition by the ethanol extract of C. speciosus are dose dependent. Therefore, this study proves that the ethanolic leaf extract of C. speciosus has enzyme inhibitory activities toward alpha-glucosidase and alpha-amylase which may helpful for the development of an antihyperglycemic formulation to reduce the postprandial glucose level. This will be carried out by bioassay-guided fractionation and isolated active compounds will be intercalated to montmorillonite, an aluminosilicate clay with high cation exchange capacity, and large specific surface area to produce sustained release formulation. Keywords: Cheilocostus speciosus, Alpha-amylase, Alpha-glucosidase, Diabetes mellitusenMaterials SciencesMineral SciencesHealth ScienceBiologyOther