Hemachandra, R.P.D.H.Priyantha, W.S.Witharane, D.D.Dassanayake, D.M.N.D.2019-06-272019-06-2720189789550481194http://erepo.lib.uwu.ac.lk/bitstream/handle/123456789/1323/03-2018-Evaluation%20of%20Test%20Cross%20Combinations%20to%20Identify%20the%20Potential.pdf?sequence=1&isAllowed=yExperiment was conducted to identify the new restores and maintainers for hybrid rice programme at Rice Research and Development Institute Batalagoda in Maha 2016/17 and Yala 2017 seasons. Hundred and forty seven crosses were produced by using 29 CMS lines crossing with 58 elite inbred lines in Maha 2016/17. All F1 hybrid progenies and respective male parents were planted in a test cross nursery in Yala 2017. Other agronomical practices were conducted according to the recommendations of the Department of Agriculture, Sri Lanka. Pollen sterility and fertility of F, hybrids were observed via light microscope after staining them with IKI solution. Fifty sixpollen fertile F1 crosses were identified having >81% pollen fertility and thirty one male parents of such crosses were selected as restorers. Meanwhile four pollen sterile F1 combinations were identified and they showed >98.6% pollen sterility (IR58025A / RES 256, IR78359A / RES 256, IR78364A / RES 256, BG CMS7A/RES 256). The pollen parent of such hybrid combinations were selected as maintainer (RES 256) and it was advanced to back cross breeding programme in order to develop new CMS lines.enAnimal SciencesAgricultureAnimal ProductionAnimal Production TechnologyOther