Gunasingha, K.R.S.S.Alwis, L.M.H.R.Kumari, H.M.P.S.Samanthi, I.A.2021-02-082021-02-0820122235-9877Potato (Solonuin luberosuin L.) is a starchy tuberous crop which belongs to the family solanaceae. Granola is a German potato variety with a high yielding and medium maturity and is recommended to grow in Sri Lanka by Department of Agriculture. In vitro induction of micro-tubers is a one type of micro propagation approach of potatoes. in vitro micro- tuberization of potato constitutes the transitory phase between in vitro multiplication end establishment of cultures in the field. They are considered as the miniature seed potatoes which represented an intermediary phase between "in vi/ro” plantlets and mini-tubers (Andreena & Champeanu, 2010). They are also called as the first generation of potsto seeds from tissue culture (Struik and Wiersena, 1999). /n vitro tuberization found to be controlled by a number of physical and chemical Factors including groMfi regulators, carbon source, photoperiod, culture conditions and genotype.(Hussey and Stacey, I984;Seabrook e/aI., 1993). Among those conditions, sucrose used as the carbohydrate and BAP (6- Benzylaminopurine) acts as the first generation synthetic cytokinins that elicit plant growth and development responses. Therefore, this study was carried out to improve the protocol for microtuber production to increase quality and quantity.Potato (Solonuin luberosuin L.) is a starchy tuberous crop which belongs to the family solanaceae. Granola is a German potato variety with a high yielding and medium maturity and is recommended to grow in Sri Lanka by Department of Agriculture. In vitro induction of micro-tubers is a one type of micro propagation approach of potatoes. in vitro micro- tuberization of potato constitutes the transitory phase between in vitro multiplication end establishment of cultures in the field. They are considered as the miniature seed potatoes which represented an intermediary phase between "in vi/ro” plantlets and mini-tubers (Andreena & Champeanu, 2010). They are also called as the first generation of potsto seeds from tissue culture (Struik and Wiersena, 1999). /n vitro tuberization found to be controlled by a number of physical and chemical Factors including groMfi regulators, carbon source, photoperiod, culture conditions and genotype.(Hussey and Stacey, I984;Seabrook e/aI., 1993). Among those conditions, sucrose used as the carbohydrate and BAP (6- Benzylaminopurine) acts as the first generation synthetic cytokinins that elicit plant growth and development responses. Therefore, this study was carried out to improve the protocol for microtuber production to increase quality and quantity.enExport AgricultureFruit and Vegetable productAgricultureOther