Perera, P.S.P.2019-04-262019-04-262012UWU/SCT/08/0047http://erepo.lib.uwu.ac.lk/bitstream/handle/123456789/313/UWULD%20SCT%2008%200047-26032019145133.pdf?sequence=1&isAllowed=yThe aim of this study was to establish maximum storage time for an intermediate storage tank by considering influenced factors which is used to store UHT standardized milk in case of breakdown. The study was carried out in 2 parts. In the first part, the quality of raw materials, line cleanliness and temperature of standardized milk in intermediate storage were investigated for 15 batch samplesas influenced factors for storage timeusing Total Plate Count (TPC), bacterial spores, yeast and molds. The obtained results were compared with standard norms (Nestle Local Norms). Temperature of standardized milk was considered as constant. In second part, to establish maximum storage time, 15 batchsamples of standardized milk were investigated using pH, conductivity and TPC initially, after 12 hours, after 24 hours, 36 hours, 48 hours and 72 hours. According to the comparison with standard norms it showed that the raw materials were microbiologically accepted for all 15 batch samples. The results from water analysis after Clean in Place (CIP) showed that there were no microbial contaminations after CIP in interior surfaces of the lines. The average pH changedeviated from recommended pH range (6.85 — 7.20) after 24 hours according to the 95% confidence interval level. For 36 hours, 95% CI level was (6.84, 6.93). Similarly, the average TPCdeviated from recommended value (105 CFU/g) after 24 hours. According to the results of 1 sample t test the average TPC in intermediate storage tankat 36 hours was greater than or equal to 105 CFU/g. The average conductivity of standardized milk at 24 hours was 1.0 — 1.5 µS/cm suggesting a recommended conductivity range for standardized milk stored in intermediate storage tank. Statistical analysis of data illustrated that maximum storage time for intermediate storage was 24 hours if the temperature maintained at 8°C with quality raw materials, and line cleanliness.enScience and Technology Degree ProgrammeThesis