Determination of Neurotoxic Pesticide Residues in Vegetables by Using Rapid Bioassay Method

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dc.contributor.authorSenanayake, O.S.
dc.date.accessioned2021-10-25T08:55:50Z
dc.date.available2021-10-25T08:55:50Z
dc.date.issued2014
dc.description.abstractPesticide residues in vegetables are determined by expensive and time consuming laboratory techniques. An experiment was conducted to develop a rapid and cost effective protocol for the analysis of pesticide residues in vegetables through Rapid Bioassay of Pesticides Residues (RBPR) method. RBPR is a method that integrates with acetyl cholinesterase (AChE) test to screen the residues of AChE inhibiting neurotoxic pesticides. In this study, 48 vegetable samples (30 non-organic from Kandy central market in Sri Lanka and 18 organics from home gardens) including cabbage, bean, and carrot were tested for pesticide residues of carbosulfan, diazinon and profenofos using RBPR. AChE was extracted from melon flies (Bactrocera cucurbitae) brain using standard protocol and its inhibition was tested. The inhibition of AChE was assessed by determining the reductions of absorbance in contaminated sample after a fixed reaction period. Calibration curves were developed for each pesticide separately and used for vegetable sample testing. Inhibition of AChE by vegetable extracts was analysed.The highest residue levels of carbosulfan,diazinon and profenofos were found in cabbage (0.00-0.53, 0.00-0.44 and 0.00-0.58 ppm, respectively) and the lowest values were recorded in carrot (0.00-0.15, 0.00-0.29 and 0.00-0.21 ppm, respectively) although the difference was not significant ( p>0.05) among vegetables. No pesticide residues were detected in organic samples. Moreover, the cost of sample testing was approximately Rs. 450.00 per sample. This bioassay technique can be used for initial screening of neurotoxic pesticide residues in vegetables as rapid and cost effective technique. Keywords: Acetyl-cholinesterase, Neurotoxic insecticide, Rapid bio assay method, Vegetablesen_US
dc.identifier.otherUWU/EAG/14/0044
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/7365/EAG%2014%20044-12022021093937.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.relation.ispartofseries;UWU/EAG/14/0044
dc.subjectExport Agriculture Degree Programme (EAG)en_US
dc.titleDetermination of Neurotoxic Pesticide Residues in Vegetables by Using Rapid Bioassay Methoden_US
dc.title.alternativeResearch Article – EAG 2014en_US
dc.typeThesisen_US
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