DNA Based Analysis for Distribution of Meloidogyne Species in Selected Crop Plants in Different Geographical Locations in Sri Lanka

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dc.contributor.authorParamalingam, D.
dc.contributor.authorHettiarachchi, G.H.C.M.
dc.contributor.authorRajapakse, R.V.D.U.P.
dc.date.accessioned2019-06-27T05:46:26Z
dc.date.available2019-06-27T05:46:26Z
dc.date.issued2018
dc.description.abstractMeloidogyne species are highly destructive endoparasitic nematodes with a wide host range. Molecular identification of the Meloidogyne species that infect crop plants was performed in this study using Meloidogyne infected 28 root samples collected from four different crops in five different geographical locations in Sri Lanka during May to July in 2017; Passara (6.934909, 81.152698) tomato, Thanamalwila (6.439755, 81.133397) chili, Pallekele (7.280104, 80.702034) capsicum, Vellankulam (9.186089, 80.126481) chili, Kaluwanchikudy (7.529373, 81.794609) chili and Kaluwanchikudy (7.529373, 81.794609) okra. Polymerase chain reactions were carried out in these samples with MF/MR primer pairs, C2F3/1108 primer pairs and 194/195 primer pairs. Genus specific universal primers MF/MR amplified, 500 by fragment of ribosomal DNA of Passara tomato, Thanamalwila chili, Pallekele capsicum, Vellankulam chili and Kaluwanchikudy chili samples representing the presence of genus Meloidogyne. Primer pair C2F3/1108 amplified, 705 by fragment of mitochondrial DNA of Thanamalwila chili, Pallekele capsicum, Vellankulam chili and Kaluwanchikudy chili samples representing the presence of Meloidogyne enterolobii. Primer pair 194/195 amplified, 700 by fragment of ribosomal DNA of Passara tomato and Kaluwanchikudy chili samples representing the presence of Meloidogyne hapla. A mixed population of M. enterolobii and M. hapla was resulted in Kaluwanchikudy chili. Kaluwanchikudy okra did not produce any band with any primer pair amplifications revealing that it had unknown species. This study confirms the expansion of the distribution of M enterolobii and M. hapla in Sri Lanka in recent years. M. enterolobii was present in all the chili/capsicum samples tested and this is the first report of its availability in infested chili/capsicum plants in Sri Lanka.en_US
dc.identifier.isbn9789550481194
dc.identifier.urihttp://erepo.lib.uwu.ac.lk/bitstream/handle/123456789/1327/07-2018-DNA%20Based%20Analysis%20for%20Distribution%20of%20Meloidogyne%20Species%20in%20.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.subjectAnimal Sciencesen_US
dc.subjectAgricultureen_US
dc.subjectAnimal Productionen_US
dc.subjectAnimal Production Technologyen_US
dc.titleDNA Based Analysis for Distribution of Meloidogyne Species in Selected Crop Plants in Different Geographical Locations in Sri Lankaen_US
dc.title.alternativeInternational Research Conference 2018en_US
dc.typeOtheren_US
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International Research Symposium of UWU-2018