Study on Effect of Growth Regulators on in-Vitro Shooting of Calathea Ornata for Commercial Cultivation
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Date
2014
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Uva Wellassa University of Sri Lanka
Abstract
Clathea ornata is a foliage plant belongs to the family Marantaceae, having high
commercial value all over the world as a house plant due its attractiveness. This study, focused to develop cost effective shoot induction protocol to obtain high number of
shoots for commercial usage. The effects of different sterilization techniques, explant types, various combination and concentration of plant growth regulators on shoot
induction were studied. The meristem explant showed high induction rate of shoots with minimum contamination percentage when explant treated with 20% (v/v) NaOC1 for 10 minutes, 0.3% HgC12 in 15 minutes and 70% alcohol 2 minutes. Further, the maximum number of multiple shoots was obtained in MS basal medium supplemented with 3.0 mg F1 6-Benzyl Amino Purine (BAP) and 3.5 mg Naphthalene Acetic Acid (NAA). Initiated shoot then sub cultured for shoot multiplication in terms of highest number of shoots and shoot length was observed in MS media in combination with BAP 3.0 mg 1-1 with NAA 3.5 mg 1-1.
Key words: sterilization, NAA, BAP, sub culture, in-vitro shoots
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Export Agriculture Degree Programme (EAG)