EFFECT OF SEED PROPAGATION PROTOCOL FOR Eucalyptus grandis

Simple item page
dc.contributor.authorRANAWEERA, R.M.C.P.
dc.date.accessioned2021-04-29T09:34:16Z
dc.date.available2021-04-29T09:34:16Z
dc.date.issued2017
dc.description.abstractEucalyptus grandis is one of the major and the most demanded plantation forest tree species, grown in up country areas of Sri Lanka. Though the seed propagation is the main mode of seedling production in commercial scale at present, drawbacks prevails due to difficulties in seed collection. In vitro propagation of Eucalyptus grandis can be enhanced the opportunities to produce elite, genetically stable, high productive and uniform planting materials, in commercial scale. The present study was aimed to establish a foundation to development of an efficient protocol for the micro propagation of Eucalyptus grandis. Seed obtained from mature surface sterilized capsules were used as the explants using a developed protocol and established under aseptic conditions. Three different tissue establishment media were used in the experiment; 1/2 MS (SG), 1/2 MS+0.5mg/L BAP (EUG) and medium contains only agar (AG). Complete Randomized Design was used with three replicates to conduct the experiment. Cultures were incubated at 25±2°C under LED light (12:12 hours at 500 lux). The percentage of germination and several morphological characteristics of the seedlings were recorded up to 60 days of inoculation. The results indicate that the most suitable media for in vitro germination of seed explants of Eucalyptus grandis is SG medium (1/2 MS) with 4.3% of germination. Continuation of further experiments with optimized conditions would help to develop a standardized protocol for in vitro propagation of Eucalyptus grandis. Keywords: In-vitro germination, BAP, Eucalyptus grandis, 1/2 MS medium, Seed explantsen_US
dc.identifier.otherUWU/EAG/13/0026
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/6647/UWULD%20EAG%2013%200026-14052019160420.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.relation.ispartofseries;UWU/EAG/13/0026
dc.subjectExport Agriculture Degree Programme (EAG)en_US
dc.titleEFFECT OF SEED PROPAGATION PROTOCOL FOR Eucalyptus grandisen_US
dc.title.alternativeResearch Article – EAG 2017en_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
UWULD EAG 13 0026-14052019160420.pdf
Size:
3.63 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Export Agriculture Degree Programme (EAG)