Determination of Sucrose, Glucose and Fructose Levels in Low Grown Black Tea Using High Performance Liquid Chromatography Technique

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dc.contributor.authorAlwis, J.A.P.N.
dc.contributor.authorPerera, G.A.A.R.
dc.contributor.authorAbeywickrama, K.R.W.
dc.date.accessioned2021-02-09T08:24:02Z
dc.date.available2021-02-09T08:24:02Z
dc.date.issued2020
dc.description.abstractNo extraneous matters are allowed to add during tea manufacture. Nevertheless, some of the manufacturers add sugar to withered tea leaves to develop better appearance in black tea. Sugar adulteration adversely affects the reputation of Ceylon Black Tea. As stipulated by the Tea Research Institute of Sri Lanka, maximum permissible levels of fructose, glucose, and sucrose for low grown black teas are 25 mg/g, 20 mg/g, and 35 mg/g respectively. The present study was conducted to determine whether the black tea manufactured in the low country in Sri Lanka was adulterated with sugar. Sample collection was done according to stratified random sampling keeping administrative districts; Galle, Matara, and Rathnapura as strata. Sixty-three black tea samples (Dust 1 grade) were collected during three consecutive weeks. Coldwater extracts of samples were prepared and their sugar levels were determined using a High-Performance Liquid Chromatograph fixed with a Zorbax NH2 column and a RI detector. The mobile phase comprised of 75% Acetonitrile and 25% de-ionized water. The average fructose, glucose, and sucrose contents of low grown tea were 6.3±2.7, 6.1±3.1, and 7.3±5.5 mg/g respectively. Fructose, glucose, and sucrose contents of all tea samples collected from Matara and Rathnapura districts were below the maximum permissible limits. Further, fructose and sucrose contents of Galle district’s black tea samples were also below the maximum permissible limits. The highest fructose, glucose, and sucrose contents were detected from the Rathnapura district’s black tea samples. It can be concluded that Matara and Rathnapura Districts’ black tea samples comply with the stipulated maximum permissible levels of fructose, glucose, and sucrose whereas Galle District’s black tea samples comply with the maximum permissible levels of fructose and sucrose. Keywords: Black tea, Fructose, Glucose, Sucrose, Adulterationen_US
dc.identifier.isbn9789550481293
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/5882/proceeding_oct_08-303.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.relation.ispartofseries;International Research Conference
dc.subjectExport Agricultureen_US
dc.subjectFood Scienceen_US
dc.subjectFood Technologyen_US
dc.subjectFood Science & Technologyen_US
dc.subjectTea Technologyen_US
dc.titleDetermination of Sucrose, Glucose and Fructose Levels in Low Grown Black Tea Using High Performance Liquid Chromatography Techniqueen_US
dc.title.alternativeInternational Research Conference 2020en_US
dc.typeOtheren_US
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