Development of a Suitable Low Cost Liquid Medium for Bio Fungicides
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Date
2015
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Uva Wellassa University of Sri Lanka
Abstract
Soil borne plant pathogenic fungi results in sever plant yield losses. Intensified use of chemical control destroys beneficial organisms and it may have undesirable health and environmental pollution risk. In order to tackle this problem, effective alternatives to chemical control are being experimented. With. this background the present study was undertaken to identify a suitable low cost base material for development of a bio fungicide and assessment for its suitability as substrates for mass multiplication Trichoderma spp.
Natural rubber serum which is rich in N, P,K, and Mg is a potential natural liquid waste that could be used to formulate a low cost suitable media for the growth of Trichoderma spp. Possibility of natural rubber serum, sakkara and their combinations were investigated at laboratory scale for formulating a new liquid medium to grow Trichoderma spp. Media that yielded the highest spore count was selected as the best combination. It was found that Trichoderma did not show a good growth neither in natural rubber serum nor in sakkara alone. Media prepared by combining rubber serum and sakkara in 3: 2 ratio gave the highest mycelial growth and yield of conidia.
It was also found that mycelial growth and spore count increased with the incubation period and maximum spore count (2.04 x 107) was given at 27th day. Optimal growth and spore count was observed when the pH of media was 8.32. Combined media which showed the best performance possess with 0.89% organic carbon, 2.46 ppm nitrogen, 200.92 ppm potassium, 7.2 ppm phosphorous and 24.47 ppm magnesium.
In mass multiplication, pH of media was lower (3.51) during the initial stage and subsequently rose to 4.65. This initial low pH affected on the growth and sporulation of Trichoderma. Highest spore count observed in mass multiplication i.e. 3.91 x 106 was significantly lower than that of the laboratory scale experiment.
Keywords: Biofungicides, sakkara, Natural Rubber Serum, Trichoderma
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Palm and Latex Technology and Value Addition Degree Programme ( PLT)