Antibacterial Activities of Endophytic Fungi of Cyperus iria Collected from Matale Distric

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dc.contributor.authorJayasundara, J.M.N.M.
dc.contributor.authorRatnaweera, P.B.
dc.contributor.authorde Silva, E.D.
dc.date.accessioned2019-07-08T08:14:13Z
dc.date.available2019-07-08T08:14:13Z
dc.date.issued2018
dc.description.abstractAntibiotic resistance of bacteria has become an ongoing severe human health concern which requires extensive research priority. Endophytic fungi of Cyperaceae family plants are considered as a potential source for isolating bioactive compounds. Hence, the objective of the current study was to isolate endophytic fungi of Cyperus iria and investigate the antibacterial activities of the crude fungal extracts. Healthy C. iria plants were collected from Weragama in Matale district and endophytic fungi were isolated from the surface sterilized roots and aerial parts using five types of media (SYP, YPD, ME, PDA and MEA) enriched with antibiotics. Each pure fungal culture was sub cultured in ten PDA dishes, incubated close to sporulation, extracted into ethyl acetate, filtered and resulting crude extracts were obtained. The crude extracts were tested for antibacterial activity using agar disc diffusion assay against four bacteria, Staphylococcus aureus (ATCC 25928), Bacillus cereus (ATCC 11718), Pseudomonas aeruginosa (ATCC 9027) and Escherichia coli (ATCC 35218) at 400 ug disc-1 concentration where Gentamycin (10 ug disc-1) and methanol (10 IA disc') were used as the positive and negative controls, respectively. Total 34 morphologically distinct putative endophytic fungi, 23 from aerial parts and 11 from roots, were isolated. Thirteen fungal extracts exhibited antibacterial activity against S. aureus, 24 against B. cereus, 12 against P. aeruginosa and one against E. coli. Among all, 29 fungi were active against at least one bacterium tested while five fungi were inactive to all. Activity of three extracts against B. cereus, 12 against P. aeruginosa, and one against E. coli was similar to the activity of the positive control. Fifteen extracts against B. cereus, 27 against P. aeruginosa, 33 against E. coli and 21 against S. aureus showed significant (p < 0.05) antibacterial activities compared to the negative control. In conclusion, C. iria from Matale harbors a lot of endophytic fungi, where several are capable of producing bioactive secondary metabolites with selective antibacterial properties.en_US
dc.identifier.isbn9789550481194
dc.identifier.urihttp://erepo.lib.uwu.ac.lk/bitstream/handle/123456789/1408/82-2018-Antibacterial%20Activities%20of%20Endophytic%20Fungi%20of%20Cyperus%20iria%20Collected%20.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.subjectBiotechnologyen_US
dc.subjectBioprocess Technologyen_US
dc.subjectBio Chemicals Engineeringen_US
dc.titleAntibacterial Activities of Endophytic Fungi of Cyperus iria Collected from Matale Districen_US
dc.title.alternativeInternational Research Conference 2018en_US
dc.typeOtheren_US
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International Research Symposium of UWU-2018