Antioxidant Activity of Selected Ten Underutilized Fruit Species

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dc.contributor.authorDilani, V.J.
dc.contributor.authorSinghalage, I.D.
dc.date.accessioned2019-07-09T09:37:48Z
dc.date.available2019-07-09T09:37:48Z
dc.date.issued2018
dc.description.abstractThe study was conducted to assess the antioxidant potential of ten underutilized fruit species namely Phyllanthus emblica L,Flacourtia sp.,Elaeocarpus serratus L., Phyllanthus acidus (L.) Skeels , Averrhoa carambola L., Averrhoa bilimbi L., Cynometra cauliflora L., Morus sp., Spondias sp. and Manilkara zapota (L.) P.Royen grown in Sri Lanka. Fruit extracts were prepared by centrifuging (4500 rpm) finely ground fresh fruit samples (20 g) with distilled water (40 ml) for 90 minutes. The extraction was repeated twice and the supernatants were used for analysis. Total Ascorbic Acid Content (TAsC) was determined using an iodomatric titration technique, calculated as mol per gram of fresh weight. Antioxidant potential was determined using DPPH• and ABTS•+ radical scavenging assays. The DPPH• Radical Scavenging Activity (RSA) of the extracts was expressed as IC50 values. The ABTS•+ activity (RSA) was calculated as percentage of discoloration. This experiment was conducted by following complete randomized design with 3 replicates. Ascorbic acid was used as a positive control and distilled water was used as a negative control. The TAsC varied among the species from 0.125x10-3 ± 0.000025 mol g- of fresh fruit of C. cauliflora L. to 4.608 x 10-3 ± 0.0001665 mol g - of fresh fruit of P. emblica L. The TAsC and antioxidant activity (DPPH• and ABTS•+ assays) were found in order to, C. cauliflora L.< Spondias sp.<A. carambola L.<Morus sp.<P. acidus L.A. bilimbi L. <E. serratus L.<Flacourtia sp.<M. zapota L.<P. emblica L. The significant antioxidant potential was possessed P. emblica L. as it showed 93.38±0.97% ABTS. +discoloration and 28.03±1.81 𝜇g ml-1 of fresh fruit needed for IC50. The lowest antioxidant potential was possessed C. cauliflora L. as it showed 1.75±1.16% ABTS. +discoloration and 381.1±3.15 𝜇𝑔m1-1 of fresh fruit needed for IC50. These results suggested that underutilized fruit species could be exploited as an ingredient in developing a potential antioxidant supplement.en_US
dc.identifier.isbn9789550481194
dc.identifier.urihttp://erepo.lib.uwu.ac.lk/bitstream/handle/123456789/1422/96-2018-Antioxidant%20Activity%20of%20Selected%20Ten%20Underutilized%20Fruit%20Species.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.subjectBiotechnologyen_US
dc.subjectBioprocess Technologyen_US
dc.subjectBio Chemicals Engineeringen_US
dc.titleAntioxidant Activity of Selected Ten Underutilized Fruit Speciesen_US
dc.title.alternativeInternational Research Conference 2018en_US
dc.typeOtheren_US
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International Research Symposium of UWU-2018