Determination of Optimal Auxin and Cytokinin Levels for Meristem Culture of Sugarcane (Saccharum Hybrid Spp.): Variety SL 96 328
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Date
2013
Journal Title
Journal ISSN
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Publisher
Uva Wellassa University of Sri Lanka
Abstract
Non-availability of quality sugarcane planting material is a major constraint for cane production
and sugar industry expansion in Sri Lanka. The Crop Improvement Division of the Sugarcane
Research Institute of Sri Lanka actively engaged in the development of protocols in quality
planting material production through micro propagation and in the other rapid sugarcane
multiplication techniques (Wijesuriya et al., 2010). Concentration of phyto-hormones has been
identified as the most critical factor that controls and interacts with the varieties in formation of
multiple shoots and root formation in culture (Wijesuriya and Teruya, 1988). The Sugarcane
Research Institute usually uses the standard MS medium with the concentration of 0.2 mg/l 6 -
Benzylaminopurine (BA) and 0.001 mg/l Kinetin (KIN) for multiple shoot formation and gelrite
medium for first culture and liquid medium for passage culture. For root formation in multiple
shoots, MS medium added with α-Naphthalene acetic acid (NAA) 0.2 mg/l, Indol-3 butyric acid
(IBA) 2 mg/l and sucrose 60 g/l have been identified as the most effective combination.
However these phyto-hormone combinations may or may not produce optimum output for
different varieties under culture. This study was conducted to determine the optimal levels of
shooting hormones (BA and KIN) and rooting hormones (NAA and IBA) in particular to the
meristem culture of newly-bred sugarcane variety SL 96 328 that is needed rapid multiplication
for commercialization.
Methodology
This experiment was conducted at the Sugarcane Research Institute, Uda Walawe, Sri Lanka
during the period, May to August 2013. Five levels of BA and five levels of Kinetin (Table 1)
were tested for their effects on multiple shoot formation and shoot multiplication in passage
culture in variety SL 96 328.
For root initiation and development in multiple shoots, five levels of NAA and five levels of
IBA (Table 2) were tested. Ten and 30 replicates were used respectively, in culturing meristem
ex-plants in gelrite medium and in passage culture of single shoots in liquid medium in each
hormone combination tested. For rooting, 20 replicates of multiple shoots with more or less
similar size and vigour were used for each hormone combination.
Description
Keywords
Agriculture, Export Agriculture, Sugarcane, Crop Production, Sugar Industry