Comparative Study on the Phytochemical Composition of Medicinally Important Three Ocimum Species Available in Sri Lanka.

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dc.contributor.authorDe Silva, S.D.A.I.
dc.contributor.authorKaliyadasa, P.E.
dc.contributor.authorLochana, E.A.L.
dc.contributor.authorMarasinghe, P.
dc.date.accessioned2021-01-19T09:09:57Z
dc.date.available2021-01-19T09:09:57Z
dc.date.issued2020
dc.description.abstractOcimum species have been used as an important medicinal herb, since ancient times but no recorded scientific evidence on morphological variations and phytochemical composition in Sri Lanka. Hence this study was conducted to determine the variation of morphological characteristics, total polyphenol content, antioxidant activity, and essential oil composition of three different Ocimum species namely; Ocimum sanctum, Ocimum gratissimum and Ocimum americanum. Healthy and fresh leaves of Ocimum plants were collected from Badulla and Welimada area. Morphological differences in leaves, flowers, roots, stem, and seeds of three Ocimum species were compared visually and recorded. Methanol extraction was performed using dried and powdered leaf samples and analysed total polyphenol content and antioxidant activity for three species using the Folin Ciocalteu reagent method and standard 2,2-diphenyl-1- picrylhydrazyl radical scavenging activity respectively. Hydro distillation was done to extract essential oils from Ocimum sanctum and Ocimum gratissimum and analysed by using Gas Chromatography – Mass Spectrometry method. There were differences and similarities between morphological characteristics among three species. The maximum polyphenol content was recorded in Ocimum americanum and the minimum was recorded in Ocimum sanctum. In determination of antioxidant activity, the highest half maximal inhibitory concentration was recorded in Ocimum americanum and the lowest was recorded in Ocimum gratissimum. There were differences in essential oil composition between Ocimum sanctum and Ocimum gratissimum. It can be concluded that there is a significant variation in morphological characteristics and phytochemical composition of three different Ocimum species available in Sri Lanka. Keywords: Antioxidant, Chromatography, Polyphenol, Scavenging, Spectrometry.en_US
dc.identifier.isbn9789550481293
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/5603/proceeding_oct_08-68.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.relation.ispartofseries;International Research Conference
dc.subjectAgricultureen_US
dc.subjectAgriculture Technologyen_US
dc.subjectAgriculture Engineeringen_US
dc.titleComparative Study on the Phytochemical Composition of Medicinally Important Three Ocimum Species Available in Sri Lanka.en_US
dc.title.alternativeInternational Research Conference 2020en_US
dc.typeOtheren_US
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