Antibacterial activity of entomopathogenic fungi isolated from a beetle (Harmonia sp.) in Sri Lanka

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dc.contributor.authorPatabedi, S.W.P.N.H.
dc.contributor.authorRatnaweera, P.B.
dc.date.accessioned2022-09-26T04:11:00Z
dc.date.available2022-09-26T04:11:00Z
dc.date.issued2021
dc.description.abstractMajority of human pathogenic bacteria has become resistant to the exsisting antibiotics causing a human health crisis in the world. Thus discovering novel antibacterial drug leads has become an urgent concern. Entomopathogenic fungi (EPF) infect insects and kill or disable them. Previous research have shown entomopathogenic fungi exhibit various bioactivities. Thus, the objective of the current study was to isolate entomopathogenic fungi from a beetle, a Harmonia sp. in Sri Lanka and evaluvate their potential of producing antibacterial compounds. EPF were isolated from surface sterilized insect cadavers collected from Balangoda area in Sri Lanka. Isolated fungi were grown in potato dextrose agar and broth media, incubated close to sporulation, harvested and extracted into ethyl aceate. The obtained weights of the crude extracts of solid and liquid cultures were compared using ANOVA. Antibacterial activity of the crude extracts were evaluated using agar disc diffusion bioassays at 400 μg/disc, against two Gram-positive bacteria, Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATTC 11778) and two Gram-negative, Escherichia coli (ATCC 35218) and Pseudomonas aeruginosa (ATCC 9027). The positive control used was Gentamycin and the negative control was methanol. Seven morphologically different EPF were isolated from Harmonia sp. The crude weights of the solid cultures were significantly higher than the crude weights of the liquid cultures (ANOVA, p < 0.05). This result showed fungi have preferred to synthesize more metabolites under solid culture conditions than liquid. Six out of the seven fungi showed antibacterial acitivities against at least one bacterium tested. EPF cultures BET 06, 08 and 10 showed high inhibition zones against the S. aureus and B. cereus. According to ANOVA there was a significant difference between the antibacterial activities of the crude extracts (p < 0.05). None of the fungal extracts showed activity against P. aeruginosa while BET 05, 06 and 08 showed mild activity against E. coli. According to the microscopic characteristics the fungi were tentatively identified as belong to Penicillium and Aspergillus species. A bioautography study reveled the presence of several bioactive compounds in BET 05, 06, 08 and 10 extracts. The results of this study showed that entomopathogenic fungi are potential sources for isolating antibacterial compounds. Keywords: Antibacterial; Entomopathogenic fungi; Harmonia sp.; Penicillium; Aspergillusen_US
dc.identifier.isbn978-624-5856-04-6
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/9659/Page%20210%20-%20IRCUWU2021%20-%20286%20-%20S.W.P.N.H.%20Patabedi%20-%20Antibacterial%20Activity%20of%20Entomopathogenic%20Fungi%20Isolated%20from%20a%20Beetle%20%28Harmonia%20sp.%29%20in%20Sri%20Lanka.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.subjectFood Scienceen_US
dc.subjectFood Science and Technologyen_US
dc.subjectFood Technologyen_US
dc.subjectAnimal Sciencesen_US
dc.subjectHealth Scienceen_US
dc.titleAntibacterial activity of entomopathogenic fungi isolated from a beetle (Harmonia sp.) in Sri Lankaen_US
dc.title.alternativeInternational Research Conference 2021en_US
dc.typeOtheren_US
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