PERFECTING EXPLANTS STERILIZATION PROCEDURE AND MULTIPLE SHOOT INDUCTION MEDIUM FOR IN VITRO PROPAGATION OF LAGENANDRA SPECIES

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dc.contributor.authorPREMATHILAKE, P. G.A.D.
dc.date.accessioned2021-03-29T07:31:18Z
dc.date.available2021-03-29T07:31:18Z
dc.date.issued2013
dc.description.abstractLagenandra species are important aquatic plants in the aquaculture industry of Sri Lanka. In the absence of a regular supply due to lack of effective propagation methods, Lagenandra species are indiscriminately harvested from the wild to supply to the export market. The threat on the species is further compounded by the loss of their rainforest habitats. Out of six Lagenandra species that occur in Sri Lanka, five are classified as "Highly threatened" species in the red list of international union for the conservation of nature. In order to overcome the problem of species loss and inadequate supply to the local and foreign markets, an in vitro micro- propagation method was developed for Lagenandra lancifolia and Lagenandra ovata. The present investigations were carried out to standardize surface sterilization of explants, evaluate different concentration of growth regulators and evaluate effect of basal media and liquid agitated culture for shoot initiation and multiplication. The study on surface sterilization of Lagenandra ovata revealed that explants treated with combination of 0.2 % mercuric chloride and 25% sodium hypochlorite, showed the highest aseptic culture establishment. Early response for sprouting and bett9r culture establishment of rhizome segments were observed on Murashige and Skoog (MS medium) medium with 5 mgl-1 BAP with presence or absence of IAA. The maximum shoot length was observed full MS media contain 5 mgl-1 BAP with 0.1 mgr 1 IAA. Though there was no leaf formation in semisolid media, 3-4 leaves per plant was observed in liquid media. Maximum number of leave were produced in liquid MS media contain 3 mgl-1 BAP with 0.1 mgl-1 IAA and 5 mgl-1 BAP . But the shoot length of liquid agitated culture was less than semi solid culture. The study on surface sterilization of Lagenandra lancifolia revealed thato, explants treated with combination of 0.2 % mercuric chloride and 20% sodium hypochlorite, showed the highest aseptic culture establishment. Shoots sprouted only on full MS liquid media contain 5 mgl-1 BAP within a period of six weeks. Key words — Explant sterilization, Growth regulator, in vitro propagation, Lagenedra speciesen_US
dc.identifier.otherUWU/AQT/09/0032
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/6273/UWULD%20AQT%2009%200032-08052019105455.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.relation.ispartofseries;UWU/AQT/09/0032
dc.subjectAquatic Resources Technology Degree Programme ( AQT)en_US
dc.titlePERFECTING EXPLANTS STERILIZATION PROCEDURE AND MULTIPLE SHOOT INDUCTION MEDIUM FOR IN VITRO PROPAGATION OF LAGENANDRA SPECIESen_US
dc.title.alternativeResearch Article – AQT 2013en_US
dc.typeThesisen_US
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