Preliminary Study on Developing Rapid Method to Detect Escherichia Coli in Food Samples

Simple item page
dc.contributor.authorPerera, B.H.K.
dc.date.accessioned2021-07-30T07:09:27Z
dc.date.available2021-07-30T07:09:27Z
dc.date.issued2016
dc.description.abstractFoodborne pathogen detection has gained much value due to it's importance regarding assuring food safety. Escherichia coli is a commonly found foodborne pathogen and it is used as an indicator bacterium for detection of fecal matter contamination. Number of methods have developed to detect E. coli in food and water. Conventional methods for detection of E. coli include culture based methods along with biochemical tests for confirmation and separation and concentration techniques. These methods have drawbacks such as high time consumption, specificity related problems and laborious nature of tests. To overcome those drawbacks, modern rapid detection methods have developed such as biosensor methods, Nucleic acid based methods, Immunological methods. They have tried to overcome above problems. But, still present problems as sensitivity and specificity related problems, complexities in use, high cost per test and resulting of false negative and positive answers. This study was carried out as a preliminary study on developing rapid method to detection of E. coli in food samples. Initially, local E. coli strain isolation and purification was done. Then confirmation of E. coli presence was done using biochemical tests. Then total protein analysis was done using SDS PAGE gel electrophoresis. Gel pattern images were analyzed. Same method was applied for Salmonella spp., Bacillus subtilis, and Pseudomans aeruginosa. Results revealed, each microbial species have different patterns of protein molecular weight distribution. This pattern can be taken within four hours. This total protein analysis method can be used to detect E. coli. But, further studies are needed for the confirmation. Key words: E. coli, Rapid detection, Food safetyen_US
dc.identifier.otherUWU/PLT/12/0021
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/6837/UWULD%20PLT%2012%200021-29042019102927.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.relation.ispartofseries;UWU/PLT/12/0021
dc.subjectPalm and Latex Technology and Value Addition Degree Programme ( PLT)en_US
dc.titlePreliminary Study on Developing Rapid Method to Detect Escherichia Coli in Food Samplesen_US
dc.title.alternativeResearch Article – PLT 2016en_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
UWULD PLT 12 0021-29042019102927.pdf
Size:
639.22 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Palm and Latex Technology and Value Addition Degree Programme ( PLT)