Perfecting Explants Sterilization Procedure and Multiple Shoot Induction Medium for In-Vitro Propagation of Lagenandra Species

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dc.contributor.authorPremathilake, P.G.A.D.
dc.contributor.authorBambaranda, M.
dc.contributor.authorJayamanne, S.C.
dc.contributor.authorKrishnarajah, S.A.
dc.date.accessioned2022-02-23T05:02:11Z
dc.date.available2022-02-23T05:02:11Z
dc.date.issued2013
dc.description.abstractLagenandra species are important aquatic plants in the aquaculture industry of Sri Lanka (Yapabandara and Ranasinghe, 2006). In the absence of a regular supply due to lack of effective propagation methods, Lagenandra species are indiscriminately harvested from the wild to supply to the export market. In order to overcome the problem of species loss and inadequate supply to the local and foreign markets, an in vitro micro- propagation method was developed for Lagenandra lancifolia and Lagenandra ovata. The maintenance of aseptic or sterile conditions is essential for successful tissue culture procedures. Various sterilization agents are used for surface sterilization the tissues. These disinfectants are also toxic to the plant tissues, hence proper concentration of disinfectants, duration of exposing the explants to the various disinfectants, the sequences of using these disinfectants has to be standardized to minimize explants injury and achieve better survival (CPRI, 1992). Mercuric chloride and sodium hypochlorite were used for the present study to standardize the best sterilization protocol for in vitro culture of Lagenandra ovata and Lagenandra lancifolia. Culture initiation and multiplication, to a great extent, are dependent on the type and genotype of explants as well as the type of hormone and their concentration. Many commercial ornamental plants are being propagated by in-vitro culture on the culture medium containing auxins and cytokinins (Peril, 2003).BAP (6- Benzylaminopurine) and IAA (Indole acetic acid) hormone with different concentration were used in the present study to perfect the best combination of IAA and BAP in shoot multiplication procedure. Methodology The research was conducted at the tissue culture laboratory, Floriculture research and development unit, Royal botanic gardens, Peradeniya. The rhizomes (1 cm - 2 cm pieces) were used as explants. In the first experiment, explants were soaked in different concentrations of sodium hypochlorite (15%, 20%, and 25%) and mercuric chloride (0.1%, 0.2%, and 0.4%) to perfecting explants sterilization procedure. Each explant was soaked for twenty minutes in Sodium hypochlorite solution and two minutes in Mercuric chloride solutions. Number of contaminated cultures was observed after two weeks from explants establishment in hormone free Murashige and Skoog medium. In second experiment, effect of different hormone combinations (hormone free MS medium, , BAP 7 mgl +IAA 0.1 mgl ) in 0.5 MS semi solid medium, full MS semi solid medium and full MS liquid medium for shoot initiation and multiplication were evaluated. Number of shoot initiation, shoot length, number of shoots per culture and number of leaves per culture were recorded after six weeks from explants establishment in MS medium.All the data were analyzed using ANOVA in SAS and Minitab statistical package.en_US
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/8423/47-AQT-Perfecting%20Explants%20Sterilization%20Procedure%20and%20Multiple%20Shoot%20Induction%20.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.subjectAnimal Sciencesen_US
dc.subjectAquaculture and Fisheriesen_US
dc.subjectFishen_US
dc.subjectfish Industryen_US
dc.subjectAquatic Resourcesen_US
dc.subjectAquatic Planten_US
dc.titlePerfecting Explants Sterilization Procedure and Multiple Shoot Induction Medium for In-Vitro Propagation of Lagenandra Speciesen_US
dc.title.alternativeResearch Symposium 2013en_US
dc.typeOtheren_US
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