Protocol for callus induction of Camellia japonica L. (Tea rose)

Simple item page
dc.contributor.authorDilrukshika, H. L. T.
dc.contributor.authorAlwis, L. M. H. R.
dc.date.accessioned2022-02-18T10:29:03Z
dc.date.available2022-02-18T10:29:03Z
dc.date.issued2015
dc.description.abstractCamellia japonica (the Japanese Camellia) is one of the best known species of the genus Camellia. Among the Camellia species, the economic value of the C. japonica ranks the highest due to its beautiful ornamental flowers, edible uses (dried flowers, oil), medicinal uses (astringent, antihaemorrhagic, haemostatic, salve and tonic) and material uses (dye, oil) (Salinero et al., 2012). Although C. japonica has a high ornamental and medicinal value, it is not popular in tea cultivating tropical agricultural country like Sri Lanka yet. Further, it was revealed that the difficulties of propagating Tea Roses are significant and therefore growers discourage to propagate them. Also C. japonica multiplication and improvement through seeds is rare due to poor seed set in the white and pink varieties present in Sri Lanka. C. japonica is usually propagated only using stem cuttings in Sri Lanka at present. But rooting was very poor in both pink and white varieties (Fernando and Alwis, 2013). But a good economic potential can be achieved in Sri Lanka due to its beautiful ornamental flower which is having long life span if it is scientifically developed to get different colors and shapes. Therefore, it is very important to in vitro propagation of C. japonica in large scale to commercially enhance its real value especially in the up country and mid country regions of Sri Lanka. Therefore this study was aimed to develop a protocol to induce the callus culture of Camellia japonica L (Tea Rose). Material and Methods This research study was conducted at Tissue Culture laboratory at Uva Wellassa University during the period of 22.04.2014 to 15.08.2014. The explants were collected from the Ury estates in Balangoda Plantations and Hakgala Botanical Garden, Hakgala, Nuwara Eliya. This study was conduct to develop an efficient protocol for rapid and prolific callus induction of Camellia japonica (Tea Rose). In the first experiment, leaves and nodal segments used as explants. Nine different combinations of 20% sodium hypochlorite for three different time durations (20 minutes, 30 minutes, 40 minutes ) and 70% ethanol for three different time durations (30 seconds, 1 minute, 1 and half minutes ) were used to select the best sterilization method. Number of contaminated vessels were counted after one week. Above nine treatment combinations were succeeded only for C. japonica leaves. Because of again used another nine different treatment combinations for surface sterilization of nodes by adjusting soaking time duration in the 20% sodium hypochlorite (35 minutes, 40 minutes, 45 minutes). In the second experiment, leaves, nodal segments and un opened flower bud flower petals used as explants. The sterilized explants were cultured on MS medium with three different hormone combinations of 3-indolebutric acid (IBA) and 6-benzylamino purine (BAP) to investigate the effect on callus induction.en_US
dc.identifier.isbn9789550481088
dc.identifier.urihttp://www.erepo.lib.uwu.ac.lk/bitstream/handle/123456789/8388/77-TEA-Protocol%20for%20callus%20induction%20of%20Camellia%20japonica%20L.%20%28Tea%20rose%29%20.pdf?sequence=1&isAllowed=y
dc.language.isoenen_US
dc.publisherUva Wellassa University of Sri Lankaen_US
dc.subjectAgricultureen_US
dc.subjectExport Agricultureen_US
dc.subjectTea Industrialsen_US
dc.subjectTea Technologyen_US
dc.titleProtocol for callus induction of Camellia japonica L. (Tea rose)en_US
dc.title.alternativeResearch Symposium 2015en_US
dc.typeOtheren_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
77-TEA-Protocol for callus induction of Camellia japonica L. (Tea rose) .pdf
Size:
447.33 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Symposium-2015