Browsing by Author "Usna, J.F."

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    Analysis of Chemical Properties and A-Amylase Inhibition of Selected Medicinal Plants for the Development Of Herbal Tea; With special reference to Cardiospermum halicacabum, Acalypha indica, Ocimum tenuiflorum, Solanum trilobatum, and Plectranthus amboinicus
    (Uva Wellassa University of Sri Lanka, 2014) Usna, J.F.
    Herbal medicines are widely used around the world because of their safety, health benefits and very less or no side effects. Further, it's known that, herbs have high content of bioactive compounds which are produced in plants through secondary metabolism. From the ancient time, the natural remedies in Siddha medicine are used to cure the non-contagious diseases such as Cancer, Diabetes and Asthma etc. However, young generation is reluctant towards herbal remedies as there are not much scientific evidences to prove their benefits. The present study attempted to identify the Chemical properties and a-Amylase Inhibition of selected herbs namely Solanum trilobatum, Ocimum tenuiflorum, Cardiospermum halicacabum, Acalypha indica and Plectranthus amboinicus and intents to develop herbal tea. Selected herbs were dried in an oven for 12 hours at 50°C and infusions were prepared by boiling 1-5 g of dried sample in 100 ml of distilled water. Infusions were tested for their Antioxidant properties using DPPH (1, 1-dipheny1-2-picrylhydrazyl) Radical Scavenging Assay and a- Amylase Inhibition using Dinitrosalicylic Acid Method and finally IC5o value was also determined. The highest level of Antioxidant Activities has been observed in Cardiospermum halicacabum (7.7 mg mrl)while it's also having maximum a-Amylase Inhibition Activity (45.5 mg m1-1). Hence, sensory evaluation was done to select the best sample for consumption (a: 0.05) and it was subjected to the chemical analysis. The phytochemical analysis confirmed that all these leaf infusions contain in between the range of Anthocyanin (0.3339±0.2361-8.4051±0.7935 mg dm-3), Polyphenol (115.2398±2.4615- 236.6486±0.3815 mg dm-3), Free Sugar (24.6727±0.5839-788.8590±34.7578 mg dm-3). Accordingly, there is a potential to develop the herbaftea by using these five herbs. Keywords: a-Amylase Inhibition, Anti-oxidant, Free Sugar, Phytochemicals, Siddha medicine
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    Analysis of Chemical Properties and α-Amylase Inhibition of Selected Medicinal Plants for the Development of Herbal Tea
    (Uva Wellassa University of Sri Lanka, 2019) Usna, J.F.; Alakolanga, A.G.A.W.
    Herbal medicines are widely used around the world because of their safety, health benefits and very less or no side effects. Further, it’s known that, herbs have high content of bioactive compounds which are produced in plants through secondary metabolism. From the ancient time, the natural remedies in Siddha medicine are used to cure the non-contagious diseases such as cancer, diabetes, asthma etc. However, young generation is reluctant towards herbal remedies as there are not much scientific evidences to prove their benefits. The present study attempted to identify the chemical properties and α-amylase inhibition of selected herbs namely Solanum trilobatum, Ocimum tenuiflorum, Cardiospermum halicacabum, Acalypha indica and Plectranthus amboinicus and intents to develop herbal tea. Selected herbs were dried in an oven for 12 hours at 50℃ and infusions were prepared by boiling 1-5 g of dried sample in 100 ml of distilled water. Infusions were tested for their antioxidant properties using DPPH (1, 1-diphenyl-2picrylhydrazyl) Radical Scavenging Assay and α- amylase enzyme inhibition using di nitro salicylic acid method and finally IC50 value was also determined. The highest level of antioxidant properties has been observed in Cardiospermum halicacabum (7.7 mg mL1) while it’s also having maximum α-amylase inhibition activity (45.5 mg mL-1). Hence, sensory evaluation was done to select the best sample for consumption and it was subjected to the chemical analysis. The phytochemical analysis confirmed that all these leaf infusions contain in between the range of Anthocyanin (0.13-8.31 mg L-1), Polyphenol (115.23-236.64 mg L-1), free sugar (24.67-236.64 mg L-1). Accordingly, there is a potential to develop the herbal tea by using these five herbs.