Browsing by Author "Sinnarasa, S."

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    Bioethanol Production from Palmyrah Molasses Using Saccharomyces Spp.omyces Spp.
    (Uva Wellassa University of Sri Lanka, 2014) Sinnarasa, S.
    Molasses is the waste material obtained from the production of palmyrah sugar candy could be used for alcohol production through fermentation process there by it reduces the production cost of sugar candy. The aim of the study was to select the suitable yeast species and optimize the fermentation conditions to produce ethanol from molasses. The best yeast strain for fermentation was selected according to their alcohol tolerance and growth on molasses using cell density. Molasses was undergone for the chemical analysis (brix, total sugar, reducing sugar, pH, total acidity and alcohol) which was used as fermentation medium .Optimization of fermentation was carried out with different initial brix (5, 10, 20, 30, 40 °Brix), inoculum size (1, 2, 3, 4 ml), pH (4, 4.5, 5, 5.5, 6, 6.5) and temperature (30, 35, 45, 55 °C), Each treatments were conducted with triplicate under controlled environment. The absorbance 1.016 and 1.576 were obtained as highest cell density per ml for 8.5 % of alcohol and growth on molasses respectively, the selected best yeast strain was used for the fermentation study. The initial brix, pH, total sugar and reducing sugar of the raw molasses was 58.73. °Brix 4.5, 62.39 and 8.06 % respectively while the alcohol content was nil. Significantly Brix 10° and inoculum size of 3 ml were selected as optimum at 3rd and 6th day of fermentation with mean value of alcohol percentage 2.6 and 4.4 respectively among the different treatment. Significantly highest alcohol percentage of 4.6, 4.8 was obtained at 5th 4th day of fermentation at optimum pH 4.5 and temperature 35 °C respectively. There were significant decreases in total solids during the fermentation of all the treatments. Bioethanol production from palmyrah molasses could be enhanced by the optimization of fermentation condition using the selected yeast strain. Key Words: Ethanol, Molasses, Palmyrah, Yeast, Fermentation
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    Optimization of Fermentation Medium for Bioethanol Production from Palmyrah Molasses
    (Uva Wellassa University of Sri Lanka, 2019-02) Sinnarasa, S.; Silva, P.D.P.M.D.; Alakolanga, A.G.A.W.; Mahilrajan, S.
    Molasses is the waste material obtained from the production of palmyrah sugar candy and it could be used for alcohol production through fermentation process thereby it reduces the production cost of sugar candy. The aims of the study were to select the best yeast species and optimize the fermentation conditions to produce ethanol. The best yeast strain for fermentation was selected according to their alcohol tolerance and growth on molasses using the cell density count in spectrophotometer. Molasses was tested for the chemical parameters such as brix, total sugar, reducing sugar, pH, total acidity and alcohol before using it as fermentation medium. Optimization of fermentation was carried out with different initial brix (5, 10, 20, 30, 40 °Brix), inoculum size (1, 2, 3, 4 ml), pH (4, 4.5, 5, 5.5, 6, 6.5) and temperature (30, 35, 45, 55 °C). Each treatment was triplicated under controlled environment conditions. Results indicated that the absorbance 0.508 and 0.788 were obtained as highest cell density for high alcohol (8.5%) tolerance and growth on molasses, respectively. The best yeast strain was used for further fermentation studies and recorded the initial brix, pH, total sugar and reducing sugar values as 58.73 oBrix 4.5, 62.39 and 8.06%, respectively while the alcohol content given nil. Brix 10o and inoculum size of 3 ml were selected as the optimum at 3rd and 6th day of fermentation with mean value of 2.6 and 4.4 alcohol percentages among the different treatments. Optimum pH for the process was recorded as 4.5 and it gave the highest 4.6 alcohol percentage on 5th day while optimum temperature was 35 °C and resulted 4.8 alcohol percentages on 4th day. There was a significant decrease in total solids of all the treatments during the fermentation process. Bioethanol production from palmyrah molasses could be enhanced up to 72% by the optimization of fermentation condition (Brix, Inoculum size, pH, Temperature) using the selected yeast strain.