Browsing by Author "Shirani, D.A."

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    Study on In-Vitro Growth, Rooting and Acclimatization of Baby’s Breath(Gypsophila paniculata L.)
    (Uva Wellassa University of Sri Lanka, 2010) De Silva, W.A.N.T.; Shirani, D.A.; Kaliyadasa, P.E.
    Baby's breath (Gypsophyla paniculata L.) is one of the important cut flower which used tissue cultured techniques to propagate commercially. Fragile nature of roots reduced in-vitro rooting and plant survival during acclimatization. Therefore, this research was conducted to improve the in-vitro rooting and plant survival of Baby's breath. Effect of four different gel strengths of MS medium (solid : 9 g/I agar, semisolid : 4.5 g/I agar, liquid and liquid with coir dust), two different aeration levels (aerated and non aerated) and two different age levels of cultures (four and six weeks aged) on in-vitro growth, rooting and acclimatization of baby's breath was studied through observing shoot height (cm), total dry weight (TDW) (g), total fresh weight (TFW) (g), no, of roots, root length (cm), no, of rooted plants by using 3 replicates and no, of survival plants observed weekly interval up to one month by using 40 plants at tissue Culture laboratory, Agricultural Research Station, Sita Eliya. Complete Randomized Design (CRD) and MINITAB statistical package were used to analyze the data. Tukey test was used to mean comparison at 5% significant level. Six weeks old plants in aerated semi solid medium was found to be better for in-vitro shooting as it recorded higher survival percentage (83.3%) than four weeks old plants in non aerated semisolid medium (50%) due to high calli growth though it was recorded the highest TDW, TDW/TFW. Six weeks old plants in aerated solid medium was recorded the best performances in in-vitro rooting which recorded 100% rooting and survival percentage with higher no, of roots (2.83) comparing to the six weeks old plants in non aerated solid medium which recorded 100% rooting and survival percentage with lower number of roots (2.16) at 5% significant level. Therefore, it was concluded that six weeks old plants in aerated semi solid medium can be used for in-vitro shooting and six weeks old plants in aerated solid medium can be used to in-vitro rooting and acclimatization. It could also be suggested that three weeks time period was sufficient for acclimatization of in-vitro grown plants. Key words: Solid, Semi solid and liquid medium, Coir dust medium, Aerated vessels, Age of in-vitro plants
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    Study on Induced MutTION nd Development of Media Protocol for Plant Regeneration from Mutated Calli of Lily(Lilium longiflorum L,)
    (Uva Wellassa University of Sri Lanka, 2010) Jayasinghe, H.A.S.L.; Shirani, D.A.; Kaliyadasa, P.E.
    The study was carried out to create novel types of Lilium longiflorum by using irradiated calli to cater the increasing demand for Lilium as one of the most important bulbous flower commercially grown in Sri Lanka. The calli were induced from lower half of the bulb scales and the basal part of the in-vitro plants in MS medium supplemented with three different combinations of BAP, IBA and 2, 4-D in the first experiment. Data were recorded on callus induction frequency, minimum days taken to appear callus, percentage of calli without direct organogenesis, growth rate and diameter of calli. In the second and third experiments, the calli were subjected to irradiation with different exposure time (10, 20, 30 and 40 minutes exposure) of UV (254 nm) and 6°Co gamma (2.5, 5 and 10 Gy) and cultured on MS medium with three different hormone combinations of BAP, NAA and GA3. Observations were made on mortality percentage of calli after irradiation, number of calli producing shoots, total number of shoots and shoot height. All the treatments were assigned in Complete Randomized Design with two factors. Each treatment of experiment 1 was consisted with 20 replicates & treatments of experiment 2 and 3 were consisted with 15 replicates. The data were analyzed using statistical package MINITAB. It was found that lower half of bulb scales cultured on MS supplemented with 0.022 mg/I BAP and 0.22 mg/I 2,4-D recorded least number of days (22 days), highest frequency of callus induction (85%), highest percentage of calli without direct organogenesis (82.35%), diameter (1.8±0.12 cm) and growth rate (3.3±0.30) at P<0.05. In Experiment 2 & 3 interaction effect of media and irradiation source was not significant at p<0.05. Irradiation levels with reduced plant height mean no of shoots and mortality less than 50%, considered as an indicator for mutations, UV with 30 minutes exposure time and 10 Gy of gamma can be selected as effective sources of mutation which recorded the lowest values for above parameters at p<0.05. At the same time mutated calli from above irradiation levels in the MS supplemented with 0.5 mg/I NAA and 0.6 mg/I BAP recorded the significant growth than other media (p<0.05). Key words: BAP, 2,4-D, NAA; Gamma radiation, UV radiation