Browsing by Author "Paramalingam, D."

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    DNA Based Analysis for Distribution of Meloidogyne Species in Selected Crop Plants in Different Geographical Locations in Sri Lanka
    (Uva Wellassa University of Sri Lanka, 2018) Paramalingam, D.; Hettiarachchi, G.H.C.M.; Rajapakse, R.V.D.U.P.
    Meloidogyne species are highly destructive endoparasitic nematodes with a wide host range. Molecular identification of the Meloidogyne species that infect crop plants was performed in this study using Meloidogyne infected 28 root samples collected from four different crops in five different geographical locations in Sri Lanka during May to July in 2017; Passara (6.934909, 81.152698) tomato, Thanamalwila (6.439755, 81.133397) chili, Pallekele (7.280104, 80.702034) capsicum, Vellankulam (9.186089, 80.126481) chili, Kaluwanchikudy (7.529373, 81.794609) chili and Kaluwanchikudy (7.529373, 81.794609) okra. Polymerase chain reactions were carried out in these samples with MF/MR primer pairs, C2F3/1108 primer pairs and 194/195 primer pairs. Genus specific universal primers MF/MR amplified, 500 by fragment of ribosomal DNA of Passara tomato, Thanamalwila chili, Pallekele capsicum, Vellankulam chili and Kaluwanchikudy chili samples representing the presence of genus Meloidogyne. Primer pair C2F3/1108 amplified, 705 by fragment of mitochondrial DNA of Thanamalwila chili, Pallekele capsicum, Vellankulam chili and Kaluwanchikudy chili samples representing the presence of Meloidogyne enterolobii. Primer pair 194/195 amplified, 700 by fragment of ribosomal DNA of Passara tomato and Kaluwanchikudy chili samples representing the presence of Meloidogyne hapla. A mixed population of M. enterolobii and M. hapla was resulted in Kaluwanchikudy chili. Kaluwanchikudy okra did not produce any band with any primer pair amplifications revealing that it had unknown species. This study confirms the expansion of the distribution of M enterolobii and M. hapla in Sri Lanka in recent years. M. enterolobii was present in all the chili/capsicum samples tested and this is the first report of its availability in infested chili/capsicum plants in Sri Lanka.