Browsing by Author "Manamgoda, D.S."
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Item Domestication of Two Edible Wild Mushrooms, Lentinus squarrosulus and Lentinus tuber-regium from Sri Lanka(Uva Wellassa University of Sri Lanka, 2019) Miriyagalla, S.D.; Silva, E.I.P.; Udayanga, D.; Manamgoda, D.S.Tropical ecosystems are rich in species diversity of wild edible mushrooms but very few domestication attempts were recorded in Sri Lanka. The objective of this study was to domesticate two wild mushroom species, L. squarrosulus and L. tuber-regium freshly collected from Sri Lanka and to evaluate suitable growth conditions for both strains. L. squarrosulus (M013) and L. tuber-regium (LSK005) were collected from Ragama and Matale respectively. Species were morphologically identified initially and to confirm the identification Internal Transcribed Spacer (ITS) region was sequenced. Sequences were verified with available sequences in GenBank and a phylogenetic analysis was performed with reference Lentinus sequences. Growth rates of the isolates were determined in four different culture media. Rice grains with 0.5% CaSO4 and corn grains with 1.2% CaSO4 and 0.3% CaCO3 were tested as mother spawn media while rubber and mango saw dust were utilized as the carbon source of the growth media. A commercially available Pleurotus ostreatus mushroom strain was used in parallel as a control in each of the above experiments. Results confirmed that the highest growth rates of both wild mushroom cultures were observed on Potato Dextrose Agar. Both the isolates colonized corn based mother spawn medium with a higher mycelial density rate. Out of the two saw dust culture media, both isolates showed a better colonization with mango. L. tuber-regium produced fruiting bodies after 70 days of inoculation of culture media whereas L. squarrosulus and commercially grown Pleurotus produced fruiting bodies after 66 and 63 days, respectively. When two culture media are compared rubber saw dust produced higher yield than mango saw dust based media. In conclusion, both wild mushroom strains, L. squarrosulus and L. tuber-regium can be successfully domesticated and are potential candidates to introduce to the consumers and commercial mushroom growers in Sri Lanka.Item Foliar Pathogenic Colletotrichum species Associated with Cultivated Rubber Trees in Sri Lanka(Uva Wellassa University of Sri Lanka, 2019) Herath, I.H.M.I.S.; Manamgoda, D.S.; Udayanga, D.Colletotrichum leaf disease (CLD) is regarded as one of the major threats on rubber trees worldwide. Due to recent revolutionary changes in species numbers in the genus Colleotrichum based on molecular data, it is essential to re-assess the Colletotrichum species associated with rubber trees in Sri Lanka. The objective of this study is to identify Colletotrichum species associated with rubber plants in Sri Lanka using morphology and DNA barcoding. Samples with leaf spots were collected randomly from rubber trees at selected sites in Colombo and Matale. Single spore isolation technique was followed to obtain five pure cultures of Colletotrichum spp. and their morphological characteristics were observed. Internal transcribed spacer (ITS) loci of all isolates were sequenced. Sequences were initially identified based on similarity search at NCBI and phylogenetic analysis was performed to further confirm the relative phylogenetic placement of Colletotrichum species. Pathogenicity tests were carried out to confirm the pathogenicity of the species and the Koch’s postulates were proven for potential pathogens. According to the morphological and molecular data, those isolates belong to C. gloeosporioides, C. gigasporium and C. truncatum species complexes. Two different species grouped within C. gloeosporioides complex showed highest growth rates in Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA) whereas C. gigasporium and C. truncatum showed the highest growth rate in PDA and MEA respectively. Typical CLD symptoms were observed with C. gloeosporioides complex species after pathogenicity testing whereas C. gigasporium and C. truncatum were also able to colonize and produce spore masses on rubber leaves. According to our knowledge this is the first record of C. gigasporium and C. truncatum associated with rubber plants in Sri Lanka. Therefore, this study reveals the unknown diversity of Colletotrichum pathogens associated with cultivated rubber trees.