Browsing by Author "Manage, P. M."
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Item Application of Newly Develop Bacterial Consortium for Decolorization of Structurally Different Textile Dyes(Uva Wellassa University of Sri Lanka, 2019-02) Ekanayake, E. M. M. S.; Udayanga, D.; Manage, P. M.Sri Lanka, niche of high quality supplier of apparel to the world, produce heavy load of textile wastewater every day. Such textile dye contained wastewater is to be treated prior discharge to the environment to maintain the pollution standards. The existing physical and chemical treatments methods are highly expensive. Therefore, the present study was focused on develop a novel bacterial consortium isolated and characterized previously as textile dye decolorizing bacteria which were isolated from textile wastewater effluent sites. Selected bacterial strains were starved overnight in 0.01 M sodium chloride, equalized the suspension at A590 = 0.35 and 5% (v/v) of suspension was introduced into sterile CI Direct Blue 201 (DB) textile dye at final concentration of 50 mg L-1. Standard spectrophotometric method was followed to determine the decolorization percentage. Three individual bacteria; Alcaligenes faecalis, Micrococcus luteus, and Staphylococcus warneri acquired 60, 64 and 72 h respectively for complete decolorization of DB dye, were selected to prepare the bacterial consortium. Decolorization of the DB dye by the bacterial consortium was completed within 48 h. It was found that the complementary interactions among three strains for rapid decolorization of DB dye than their individual effect. Decolorization of DB dye by the consortium was further enhanced under static conditions with the presence of yeast extract and glucose in the medium. Repetitive addition of DB dye to the same initial biomass showed a complete decolorization up to four cycles and descending decolorization trend was observed afterward. Further, the bacterial consortium was able to complete decolorization of selected structurally different textile dyes (Vat green FFB, Cibracorn blue, Moxillon blue) confirming their application on treatment of wide range of textile dyes by providing a greener approach to Sri Lankan textile dyeing industry to fulfill 2030 Green Environment Concept.Item Contamination Status of Pathogenic Bacteria and Water Quality of Groundwater in Angunukolapelessa, Sri Lanka(Uva Wellassa University of Sri Lanka, 2019-02) Mahagamage, M.G.Y.L.; Abinaiyan, I.; Manage, P. M.Waterborne infections are common and widespread when clean water and sanitation are poor. In Sri Lanka, 2588 Salmonella positive cases have been recorded from 2005 to 2013. Therefore, the present study was carried out to evaluate the chemical contamination status of 70 well water sources in Angunukolapelessa area where only 30 wells were subjected to microbial analysis to cover Angunukolapelessa divisional secretariat division during October 2018. Total coliform, faecal coliform, Salmonella spp. and Shigella spp. were screened along with some physico-chemical parameters of groundwater. Sampling, transportation and analysis were done following the standard protocoles. Statistical analysis was done and thematic maps were prepared using ArcGIS software. Results of the study revealed that entire area was contaminated with both total and faecal coliform bacteria and the values were not within the SLS and WHO drinking water quality standards. Twenty percent of collected samples were positive for Salmonella spp. and most of the locations are being used to extract water for drinking purposes. However, Shigella spp. was not recorded during the study period. Seventy-one percent of sampling locations were recorded as hard water (121 mg L-1<) and around 16% of samples were not within the guideline values given by the SLS drinking water quality for Electrical Conductivity (EC). The minimum and maximum of pH and flouride concentration was recorded as 6.99 - 8.62 and 0.05 to 1.97 mg L-1 respectively where the highest flouride concentration was recorded from Daha amuna location. The tested other water quality parameters; N-NO2, N-NH3 and total phosphate (TP) concentrations were recorded within the Sri Lanka drinking water standards.