Browsing by Author "Herath, T.N.B."

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Development of a Ready to Eat Canned Fish Using Underutilized Fish with Different Filling Materials
    (Uva Wellassa University of Sri Lanka, 2019) Sathsarani, D.W.N.; Udayathilaka, K.E.; Herath, T.N.B.; Abeyrathne, E.D.N.S.
    Fishes are one the most important nutrient sources for humans. Nowadays the demand for ready to eat food is increasing. Present study was to develop a ready to eat canned fish by using underutilized fish. Two fish species, Frigate tuna and Rainbow runner were used and preliminary trials were conducted to find out the best spices level (chili powder, pepper, coriander, garlic, turmeric, cinnamon, ginger, brindle berry cream, cardamom, curry leaves, pandan leaves) and filling solutions (tomato sauce, soy sauce, soybean oil and water) followed by sterilization at 121ºC for different times (50, 65, 70, 75 minutes) using 30 untrained panelists. Colour, texture, aroma, juiciness, mouth feel, overall taste and overall acceptance tested were analyzed by using the 9 point hedonic scale. Best combination of species and filling solutions were subjected for keeping quality analysis by checking pH, lipid oxidation (TBARS and DPPH) and microbial count (TPC, E. coli and Salmonella). According to the sensory data, soy sauce and tomato sauce were selected as the best filling solutions for Frigate tuna and Rainbow runner (p<0.05). Rainbow runner was better in all sensory attributes in tomato sauce and soy sauce compared to the rest (p<0.05). Sterilization for 65 minutes at 121ºC was selected as the best sterilization condition in comparing organoleptic properties (p<0.05). Canned fish produced by Rainbow runner with tomato sauce and soy sauce were selected for keeping quality analysis. Final products were free from E. coli and Salmonella. pH of the soy sauce and tomato sauce range from 5.65±0.19 to 5.75±0.01 and 5.59±0.10 to 5.64±0.11, respectively during storage. Lipid oxidation and TPC were within the acceptable levels during the storage period for 30 days at room temperature. Proximate analysis confirmed that the product is rich in protein. As the conclusion, Rainbow runner fish can be used as one of the best underutilized fish species to produce ready to eat canned products.
  • Thumbnail Image
    Item
    Preliminary Screening of Marine Algal Species for Isolation of Bioactive Compounds from Caulerpa racemosa, Sargassum crassifolium and Ulva reticulata
    (Uva Wellassa University of Sri Lanka, 2019-02) Samarakoon, D.M.B.K.; Thiruchenduran, S.; Herath, T.N.B.
    Phytochemicals have been proved to be a reliable pharmaceutical source having beneficial biological activities for the treatment ailments in traditional medicines. In this context, seaweed species have been concerned as one of the important phytochemical sources. The current study explored three seaweed species, Caulerpa racemosa, Sargassum crassifolium and Ulva reticulata. Samples were collected from the southern coast of Sri Lanka and identified to the species level using species keys for macroalgae. The samples were processed to remove foreign material and shade dried prior to two different extraction protocols. As a preliminary step for the identification and isolation of target compounds, the extracts were driven through different screening protocols. Extraction protocols were followed for the phytochemical screening and DPPH scavenging activity separately. Sequential extraction was followed for the assessment of antioxidant activity. Terpenoids, steroids, phenolic compounds, flavonoids, saponins and alkaloids were present in all three species in detectable levels. Considering DPPH radical scavenging activity, methanolic extract of C. racemosa has shown significantly higher activity with a mean of 54.41% and methanolic extract of U. reticulata has shown a significantly lower activity with a mean of 36.76%. Methanolic extracts of C. racemosa and S. crassifolium and ethyl ether extracts of U. reticulata and S. crassifolium have shown significantly higher radical scavenging activity. Water has shown poorer radical scavenging activity for all three species. Based on the results obtained, all three species have active compounds. Polar fractions of C. racemosa and S. crassifolium and non-polar fractions from U. reticulata suitable to use for isolation of bioactive compounds. It is suggested to carry out isolation activities for significantly higher activity fractions of the extracts which could be used to identify appropriate solvent extraction.