Browsing by Author "Herath, H.M.B.M.M.D."

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    Screening for Antibacterial Potential of Marine Algae Extracts from West Coast of Sri Lanka
    (Uva Wellassa University of Sri Lanka, 2019-02) Herath, H.M.B.M.M.D.; Yapa, P.N.
    Marine algae are produced wide variety of bioactive compounds and some of them can be used to development of antibacterial novel drugs. The present study was aimed to evaluate antimicrobial activity of some marine algae extracts from west coast of Sri Lanka. Pure cultures of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis and Pectobacterium carotovorum were used as the test microorganisms for antibacterial testing. Marine algae such as Ulva prolifera, Chaetomorpha antennina, Cladophoropsis sundanensis, Sargassum crassifolium, Asteronema breviarticulata, Chnoospora minima, Gracilaria multipartita, Gracilaria folifera, Gracilaria hikkaduwensis, Rhodopeltis sp., Grateloupia lithophila and Laurencia natalenis were collected from Kalpitiya sea coast. They were freshly ground and 25 g of each fresh algal sample was extracted in 50 ml of distilled water, methanol, acetone, diethyl ether separately and concentrated to 0.5 g/ml following solvent extraction at 30 oC. The test bacteria of 108 CFU/ml were introduced and uniformly spread on the surface of Muller Hinton agar plates separately and followed standard agar –gel diffusion inhibition procedure. After placing 25 µl of extracts into each well, plates were incubated at 37 °C and 30 oC respectively for 24 hours and the diameter of the growth inhibition zone around the wells were measured. Comparisons were performed using one-way ANOVA followed by Duncan multiple-range test. It was revealed that the all tested extracts of C. minima showed antibacterial activity against S. aureus and all extracts of G. folifera against E. faecalis. Methanolic, acetone and diethyl ether extracts of G. hikkaduwensis inhibited the growth of S. aureus. Further, methanolic extracts of A. breviarticulata, S. crassifolium and U. prolifera showed inhibitory effect against all tested bacterial species. Finally, it can be concluded marine algae from Kalpitiya coast of Sri Lanka are potential sources of bioactive compounds and should be investigated for identifying natural antibiotics.