Browsing by Author "Gimhani, D.R."

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    Analysis of Allelic Diversity of Drought Responsive ERF Genes in Sri Lankan Rice Germplasm
    (Uva Wellassa University of Sri Lanka, 2021) Wehalle, W.G.A.D.N.; Gimhani, D.R.; Abhayawickrama, B.P.
    Ethylene response factor (ERF), is a prominent transcription factor gene family that governs numerous abiotic stress-related physiological activities in plants. Drought is the foremost yield- limiting factor of the second-largest staple crop of the globe. Bioinformatics tools provide a robust platform to investigate the genetic characteristics towards rice plant drought responses. The present study aimed to analyze the allelic variations of OsEBP89, OsERF101, OsERF71, OsERF48, OsERF12 and OsLG3; drought-responsive ERF genes in silico within the Sri Lankan rice pool. Genes were selected from the Rice Annotation Project (RAP) database concerning Oryza sativa sp. japonica cv. „Nipponbare‟ as the reference genome. Selected gene sequences of 47 Sri Lankan rice varieties were retrieved from the Rice SNP-Seek database and allelic variation was assessed concerning the corresponding gene sequences of Nipponbare using MEGA 7- Clustal W method. Present Results revealed several InDels and SNPs: 6 SNPs in OsEBP89 (A/G at 15nt, G/T at 380nt, C/T at 381nt, T/A at 555nt, C/T at 709nt and G/T at 951nt), 1 InDel and 3 SNPs in OsERF101 (C/A at 87nt, A/G at 687nt and C/T at 725nt), 8 SNPs in OsERF71 (C/A at 118nt, G/A at 360nt, C/T at 623nt, G/A at 742nt, G/T at 849nt, C/T at 867nt, T/C at 939nt and G/A at 981nt), 1 InDel and 5 SNPs in OsERF48 (C/G at 655nt, G/Tat 768nt, C/G at 812nt, C/T at 1112nt and G/C at 1162nt), 1 InDel and 5 SNPs in OsERF12 (G/T at 41nt, C/T at 150nt, G/A at 336nt, C/G at 365nt and C/T at 957nt) and 3 SNPs in OsLG3 (C/T at 293nt, C/T at 487nt and A/G at 810nt).The MEGA 7 assisted phylogenetic analysis unveil apparent allelic variation within the Sri Lankan rice germplasm. The encoded proteins of OsERF101, OsERF48 and OsERF12 genes were subjected to secondary structure prediction and physicochemical analysis. The Physicochemical properties varied among structures of corresponding proteins. However, major differences in folding patterns were not observed. Functional characterization and phenotypic validations are essential to investigate the potential of these genes in drought-tolerant rice improvement programs. Keywords: Allelic variation; Drought stress; Ethylene Response Factor; Rice
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    Evaluation of Anti-oxidant Properties of Four Sri Lankan Traditional Red Rice Varieties
    (Uva Wellassa University of Sri Lanka, 2021) Perera, D.A.U.H.; Thilakarathna, H.R.M.G.C.; Gimhani, D.R.; Wijesinghe, J.A.A.C.; Kottearachchi, N.S.
    Rice grains with red pericarp tend to present relatively higher antioxidant activity than those with light brown pericarp colour because of their rich anthocyanin and proanthocyanidin content. Currently, large numbers of local rice breeders and consumers are interested in red rice varieties due to their potential health benefits provided through strong antioxidant properties. The current study aimed to assess the antioxidant properties of four selected traditional red rice varieties in Sri Lanka, namely, Pachchaperumal, Kaluheenati, Suduheenati and Madathawalu. The extracts obtained from rice brans were examined for their total phenolic content, DPPH radical scavenging activity, total proanthocyanidin content, total flavonoid content and total anthocyanin content. Total phenolic content was examined in both soluble and bound fractions using the Folin–Ciocalteu‟s method. DPPH radical scavenging activity from bound fraction was assessed using 0.1 mM DPPH solution. The results were analyzed using One-Way Analysis of Variance (ANOVA) followed by Turkey‟s pair-wise test as the post-hoc analysis. The amounts of phenolics in the soluble fraction were much higher than those in the bound fraction of these four red rice samples. According to the results, Kaluheenati showed the significantly highest value (p < 0.05) for total phenolic content (soluble: 54.49 mg gallic acid equivalents /100g; bound: 24.31 mg gallic acid equivalents /100g) and total proanthocyanidin content (138.15 mg catechin equivalent /100g), while Madathawalu showed the significantly highest (p < 0.05) value for DPPH radical scavenging activity (73.5 %) and total anthocyanin content (24.57 mg cyanidin-3-glucoside equivalents /100g). The highest value for total flavonoid content was examined in a variety of Pachchaperumal and it was 183.59 mg quercetin equivalents/100g. According to the present results, it could be speculated that Pachchaperumal may contain a higher amount of other flavonoids compared to anthocyanins. And also the promising contribution of total anthocyanin content and proanthocyanidin content for higher antioxidant activity was indicated by the present results. In conclusion, according to the present study, both Kaluheenati and Madathawalu exhibited the highest overall antioxidant properties, than the other tested varieties. Further studies are required to conduct with increased sample sizes along with different extraction systems, to explore the nutritional values. Keywords: Anthocyanins; Antioxidant; Proanthocyanidins; Traditional red rice