Browsing by Author "Dissanayake, Y."

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    Identification of Vectors for In-vivo Protein Overexpression in Introducing Drought Resistance: A Review
    (Uva Wellassa University of Sri Lanka, 2019) Cooray, R.; Dissanayake, Y.; Warnakula, L.; Hapuarachchi, N.S.
    The prolonged lack of rainfall is a major abiotic stress affecting crop productivity in Sri Lanka. In vivo protein overexpression to induce drought resistance is frequently studied globally however; the selection of a most suitable vector for gene transfection has been a challenge for high protein yield. This study aims to review such possible vectors, through a thorough published literature survey at ‘Google scholar’ and ‘PubMed’ with search terms ‘drought resist*’, ‘drought tolerant*’, ‘vector’, ‘overexpression’. A late embryogenesis abundant protein gene OsLEA3-1 overexpressed with three binary expression constructs (OsLEA3-S/A/H), by inserting the full length cDNA into backbone vectors pCAMBIA1301-S/A/H respectively, with double CaMV 35S, rice Actin1 and HVA1-like promoter, followed by Agrobacterium transformation. All other constructs OsLEA3-S/-H had higher grain yield than wild type under stress except OsLEA3-A. Over expression levels are 63% 56% and 46% for OsLEA3-S/A/H, respectively. Protein coding region of OsbZIP72 in vector pCAMBIA1300S to construct pCAMBIA1300S-OsbZIP72; and a promoter of drought tolerance rice cultivar IRAT109 in a pCAMBIA1381xb-GFP vector to control GFP expression and Arabidopsis BnLEA4-1 in pGEMT-BnLEA4-1 vector transfected via Agrobacterium are few examples at global scale. However, no published studies in Sri Lanka were found during the literature search. Therefore, cloning of drought tolerant gene to a crop plant followed by in vivo overexpression to stimulate the drought tolerance is a promising biotechnological advancement in agriculture. Literature showed vectors can be modified to fit to an individual crop product for in vivo overexpression of drought tolerant genes. It is recommended to extend the review to analyse characteristics for a suitable vector include compatibility, linker region, and screening genes to develop a complete guide for selection of vector in in vivo protein overexpression for drought resistance.
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    Importance of Genetic Diversity and Phytochemical Assessment of Madhuca spp. in Sri Lanka: A Review
    (Uva Wellassa University of Sri Lanka, 2019) Withana, W.V.E.; Hapuarachchi, N.S.; Cooray, R.; Dissanayake, Y.; Warnakula, L. W
    Madhuca is a large evergreen tree belonging to the family Sapotaceae distributed in many Asian countries including Sri Lanka. The aim of this review was to identify potential applications of Madhuca spp.in Sri Lanka to be developed into a research prospective in genetic and phytochemistry analysis. Literature survey was done using “Google Scholar” search engine and “PubMed” database using search terms “Madhuca”,” Genetics”,” Phytochemistry”. Altogether twenty-two research studies were retrieved on phytochemical analysis, pharmacological profiles but fewer in its other utilizations and genetic analysis. Five research articles were found on phytochemistry studies and three articles were about modified DNA isolation methods and genetic diversity analysis using Madhuca spp. in India. Studies showed that all parts of the Madhuca tree carry a number of medicinal properties and is rich in secondary metabolites. Literature evidence depicted it as a multipurpose forest tree, source of food and nutrition, pharmaceutical ingredient, bio-fertilizer and bio-fuel. This search identified seven Madhuca species currently found in Sri Lanka of which four of them are endemic. Being a plant species with a wide scope of potential applications, to date there are only one research available in published literature on phytochemical analysis of four Madhuca species found in Sri Lanka. None of supporting materials were found on genetic studies in genus Madhuca in Sri Lanka. Hence this raises the demand for such phytochemical analysis of different Madhuca spp. in Sri Lanka. Developing methods to extract genetic data, molecular authentication studies via molecular barcoding to fill the gaps in phenotypic and genotypic characterisation, storing the genetic data in databases for public access for future research purposes and efficient sustainable germplasm management will widen the research scope and potential application development of this invaluable plant in Sri Lanka.