Browsing by Author "Dilrukshi, P.A.D.M."

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Antimicrobial Activity of Ageratum Conyzoides Against Staphylococcus Aureus
    (Uva Wellassa University of Sri Lanka, 2013) Dilrukshi, P.A.D.M.
    Ageratum conyzoides is used in traditional medicine for the treatments of variety of alignment. The aim of this research was to formulate and evaluate the wound healing activity of methanolic extract of Ageratum conyzoides. Staphylococcus aureus is most common bacteria in wounds. The antibacterial activities of Ageratum conyzoides was investigated against Staphylococcus aureus, using the Well diffusion method. The solvent type extracts were obtained by soxhlet method with methanol. Solvent was removed by using rotary evaporator at 150rpm below 40°C and obtained semi solid slurry. Stock solutions were prepared by dissolving semi solid slurry with methanol. These were tested in varying concentration of 200mg/ml — 12.5mg/ml. The solvents were used as negative control. Solvents had no effect on the microorganisms. Each parts of Ageratum conyzoides such as leaves, flowers, roots and stems showed antimicrobial inhibitory activity at 200mg/ml. Highest inhibition zone was showed leaves of Ageratum conyzoides and inhibition zone was decrease when concentration decrease. MIC of the Ageratum conyzoides leaves extract is 12.5mg/mL determine through concentration series. This study suggests that the methanol extracts of Ageratum conyzoides, can be used as herbal medicines in the control of Staphylococcus aureus. Methanolic extract of Ageratum conyzoides leaves was used to formulate the herbal ointment. Formulations were then tested for its physicochemical properties. Further, herbal formulations were evaluated for its 'anti- bacterial activity against Staphylococcus aureus by agar well diffusion method using Betadine as the standard. The formulations showed predominant activity against selected species. This study showed that the leaf extracts of the Ageratum conyzoides plant, had good antibacterial activity against Staphylococcus ,aureus. Prepared herbal ointment also showed antibacterial activity against Staphylococcus aureus. That ointment had more than 50% consumer preference and didn't show any allergy reaction. Overall result of this study reveals that this is an effective herbal ointment. Key words: Ageratum conyzoides, Staphylococcus aureus, Well diffusion method, antibacterial activities.
  • No Thumbnail Available
    Item
    Antimicrobial Activity of Ageratum conyzoides against Staphylococcus aureus
    (Uva Wellassa University of Sri Lanka, 2013) Dilrukshi, P.A.D.M.; Premathilake, S.N.; Wijesekara, K.B.
    Ageratum conyzoides is a native annual branching herb, which grows to approximately 1 m in height and usually found open and abandoned areas . This plant is commonly used in traditional medicine, especially for wound healing (Sing et al., 2012). Methodswhich are usedinhealing wound infections include debridement, antibiotics, tissue grafts and proteolytic enzymes. However, these methods have major drawbacks and unwanted side effects. Recently there is a tendency towards the uses of traditional medicines as it shows the better cultural acceptability and better compatibility with the human body and also fewer side effects (Parekh et al., 2005). Fresh plant materials of A.conyzoides were collected and washed using tap water .Then they were separated into flowers, leaves, roots and stems and were air dried in shade for 7 days and powdered using mortar and pestle. The powdered plant materials were sieved and stored in airtight containers. Plant material extracts were obtained with 95% methanol using the soxhlet apparatus. Each extracts were concentrated and solvents were fully evaporated, by rotary evaporator (150 rpm) at 40 C. The obtained concentrates were transferred to McCarthy glass vials and placed under room temperature for complete dryness. Then they were stored in airtight vials under refrigerated conditions. Staphylococcus aureuspure cultures were collected from the Medical Research Institute (MRI) Colombo and from them liquid cultures were prepared using Nutrient broth. Then they were incubated at 37°C for 24 hrs. The powdered plant materials were measured into separate McCartney bottles and appropriate volume of the extracts were added to make a stock solution of 200mg/mL. Sterile nutrient agar plates were prepared and allowed to solidify. A 0.1 mL of liquid culture of S. aureuswas spread equally on the solidified nutrient agar plate. After one hour five wells were dug in each plates using a sterile cork borer (5mm diameter).Concentration series of extracts (200mg/mL, 100mg/mL,50 mg/mL, 25mg/mL and 12.5 mg/mL) were prepared and from them 0.5mLof extracts were added to wells in appropriately labelled plates. As the control 95% methanol was used. The plates were left on the bench for few minutes for the extract to diffuse into the agar and later incubated at 37°C for 24hours. After the incubation the zone of clearance around each well was measured using a metric ruler by taking measurement of the inhibition zones. Minimum inhibition concentration (MIC) was determined for extracts that showed less than 7 mm(<7 mm) diameter inhibition zone.