Browsing by Author "Ariyarathne, H.B.S."

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    Investigation of Genetic Variation in Bmp4 Gene in Local Indigenous and Jamnapari Crossbred Goats in Damana Veterinary Service Division Sri Lanka
    (Uva Wellassa University of Srilanka, 2011) Wijesena, H.R.; Bulumulla, P.B.A.I.K.; Lokugalappatti, L.G.S.; Ariyarathne, H.B.S.
    Small ruminants, such as goats (Capra hircus), constitute an important livestock resource in most countries and are essential for the livelihood of many farmers (Baker et al., 2003). Application of molecular genetics approaches for the genetic progress of quantitative economic traits such as growth and reproduction in goats is an effective way of increasing their production as these methods could lead to finding of genetic markers useful for improved selection. Molecular genetics approaches have been used in the world for goat production in the recent past, and these strategies are yet to be established in Sri Lanka since they require high knowledge and capital investments. Therefore, this study was conducted as a preliminary step for the application of molecular genetics approaches in selection of goats for improved production in Sri Lanka. Single Stranded confirmation Polymorphism (SSCP) analysis is one such powerful genetic screening method to identify the sequence variation in Polymerase Chain Reaction (PCR) amplified products. In the present study, we investigated the PCR-SSCP genetic variation in the intron 2 of Bone Morphogenetic Protein 4 (BMP4) gene, which plays a major role in growth and reproduction. The study was focused on Local types (LT) and Jamnapari crossbred (JC) goats in Damana Veterinary Service (VS) division in the Ampara district of Sri Lanka.